SERCA1 ATPase Overexpression Lysate

Western Blot: SERCA1 ATPase Overexpression Lysate (Adult Normal) [NBP2-05144] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SERCA1 ATPase.

• Reactivity: Hu
• Applications: WB

SERCA1 ATPase Overexpression Lysate Summary

• Description:

  SERCA1 ATPase Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC214911
  
  Accession#: NM_173201
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: ATP2A1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 110.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for SERCA1 ATPase Overexpression Lysate

• ATP2A
• ATP2A1
• ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
• Calcium pump 1
• Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletalmuscle isoform
• EC 3.6.3
• EC 7.2.2.10
• Endoplasmic reticulum class 1/2 Ca(2+) ATPase
• sarcoplasmic/endoplasmic reticulum calcium ATPase 1
• SERCA1 ATPase
• SERCA1
• SERCA1EC 3.6.3.8
• SR Ca(2+)-ATPase 1

Background

SERCA1 ATPase encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ATP2A1