Septin-5 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Septin-5 Antibody [NBP2-48588] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Septin-5 Antibody [NBP2-48588] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: Septin-5 Antibody [NBP2-48588] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: Septin-5 Antibody [NBP2-48588] - Staining in human cerebral cortex and liver tissues using anti-SEPT5 antibody. Corresponding SEPT5 RNA-seq data are presented for the same tissues.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Septin-5 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: QQMTSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDEELRRMQEMLQRMKQ
• Predicted Species: Mouse (98%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEPTIN5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Septin-5 Recombinant Protein Antigen (NBP2-48588PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Septin-5 Antibody - BSA Free

• CDCREL
• CDCREL1
• CDCREL-1
• cell division control related protein 1
• Cell division control-related protein 1
• HCDCREL-1
• peanut-like 1 (Drosophila)
• peanut-like 1
• Peanut-like protein 1
• platelet glycoprotein Ib beta chain
• PNUTL1H5
• septin 5
• septin-5

Background

This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Two transcripts of this gene, a major one of 2.2 kb and a minor one of 3.5 kb, have been observed. The 2.2 kb form results from the utilization of a non-consensus polyA signal (AACAAT). In the absence of polyadenylation from this imperfect site, the consensus polyA signal of the downstream neighboring gene (GP1BB; platelet glycoprotein Ib) is used, resulting in the 3.5 kb transcript. An alternatively spliced transcript variant with a different 5' end has also been identified, but its full-length nature has not been completely determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEPTIN5