Septin-3 Antibody

Images

 
Western Blot: Septin-3 Antibody [NB100-1023] - (1ug/ml) of Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Septin-3 Antibody Summary

Immunogen
Peptide with sequence SELVPEPRPKPA-C corresponding to N-Terminus according to NP_663786.1, NP_061979.3.
Specificity
This antibody is expected to recognize both reported isoforms according to NP_663786.1 and NP_061979.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SEPT3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 40 kDa band observed in human brain lysate.
IHC: Paraffin embedded Human Brain (Cortex) and (Cerebellum) and Testis.
Positive Control
Septin-3 Lysate (NBL1-07141)
Brain Lysate (NB820-59177)
Publications
Read Publication using NB100-1023.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Septin-3 Antibody

  • bK250D10.3
  • MGC133218
  • neuronal-specific septin-3
  • SEP3neuronal-specific septin 3
  • septin 3

Background

This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Septin-3 Antibody (NB100-1023)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEPT3
Entrez
Uniprot