Septin-3 Antibody

Western Blot: Septin-3 Antibody [NB100-1023] - NB100-1023 (0.5ug/ml) staining of Human Cerebellum (A), (0.1ug/ml) Mouse (B) and (0.03ug/ml) Rat (C) Brain lysate (35ug protein in RIPA buffer).. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Septin-3 Antibody [NB100-1023] - staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Septin-3 Antibody Summary

• Immunogen: Peptide with sequence SELVPEPRPKPA-C corresponding to N-Terminus according to NP_663786.2, NP_061979.3.
• Specificity: This antibody is expected to recognize both reported isoforms according to NP_663786.2 and NP_061979.3.
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEPT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03 - 0.5 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in Human Cerebellum and in Mouse and Rat Brain lysates (calculated MW of 40.7kDa according to NP_663786.2 and NP_062248.2, and 40.6kDa according to NP_001345765.1)
• Control:

  Septin-3 Overexpression Lysate
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Septin-3 Antibody

• bK250D10.3
• MGC133218
• neuronal-specific septin-3
• SEP3neuronal-specific septin 3
• septin 3

Background

This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Septin-3 Antibody (NB100-1023)(1)

Publication using NB100-1023

• Xue J, Tsang CW, Gai WP et al. Septin 3 (G-septin) is a developmentally regulated phosphoprotein enriched inpresynaptic nerve terminals. J Neurochem 2004-11-01 [PMID: 15485489]

Bioinformatics

• Gene Symbol: SEPT3
• Entrez:
  • 55964(Human)
  • 24050(Mouse)
• Uniprot:
  • NP_061979(Human)