Septin-2 Antibody

Western Blot: Septin-2 Antibody [NBP1-06076] - Staining of Human Testis lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Cortex. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Breast tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-06076] - Staining of paraffin embedded Human Tonsil tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Septin-2 Antibody Summary

• Immunogen: Peptide with sequence SKQQPTQFINPET-C corresponding to N-Terminus according to NP_001008491.1.
• Epitope: SKQQPTQFINPET-C
• Specificity: Reported variants represent identical protein: NP_001008492.1, NP_006146.1, NP_004395.1, NP_001008491.1.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEPTIN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.05 - 0.2 ug/mL
• Application Notes: Western blot: Approx. 40kDa band observed in Human Testis lysates (calculated MW of 41.5 kDa according to NP_001008491.1). Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Breast, Tonsil and Brain (Cortex).
• Control:

  Septin-2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Septin-2 Antibody

• DIFF6NEDD-5
• hNedd5
• KIAA0158Neural precursor cell expressed developmentally down-regulated protein 5
• NEDD5Pnutl3
• neural precursor cell expressed, developmentally down-regulated 5
• septin 2
• septin-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Septin-2 Antibody (NBP1-06076)(1)

Publication using NBP1-06076

• Kremer BE, Adang LA, Macara IG. Septins regulate actin organization and cell-cycle arrest through nuclearaccumulation of NCK mediated by SOCS7. Cell 2007-09-07 [PMID: 17803907]

Bioinformatics

• Gene Symbol: SEPTIN2
• Entrez:
  • 4735(Human)
  • 18000(Mouse)
  • 117515(Rat)
• Uniprot:
  • NP_001008491.1(Human)