Secretogranin 3 Overexpression Lysate (Native)

Images

 
Western Blot: Secretogranin 3 Overexpression Lysate (Adult Normal) [NBL1-15730] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Secretogranin 3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Secretogranin 3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203080 and based on accession number NM_013243. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens secretogranin III (SCG3), mRNA.
Gene
SCG3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
50.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Secretogranin 3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Secretogranin 3 Overexpression Lysate (Native)

  • secretogranin IIIFLJ90833
  • secretogranin-3
  • SGIII

Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Granins may serve as precursors for biologically active peptides. Some granins have been shown to function as helper proteins in sorting and proteolytic processing of prohormones; however, the function of this protein is unknown. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-15895
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB120-15160
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NBP2-50288
Species: Rt
Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
AF3587
Species: Hu
Applications: WB, IHC, IP, CyTOF-ready, ICC, ICFlow
NBP1-80782
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-46535
Species: Hu, Mu, Rt, Rb, Xp, Ze
Applications: WB, IHC, IHC-Fr, IHC-P
NBP2-52432
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready, ICC
AF6018
Species: Hu, Mu
Applications: WB, IP, ICC
NBP2-29421
Species: Hu, Rt, Po, Bv, Gt
Applications: WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NBP1-80944
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-14924
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
H00027344-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
H00005122-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NB110-60935
Species: Hu, Mu, Pm
Applications: WB
NBP1-31078
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Secretogranin 3 Lysate (NBL1-15730) (0)

There are no publications for Secretogranin 3 Lysate (NBL1-15730).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Secretogranin 3 Lysate (NBL1-15730) (0)

There are no reviews for Secretogranin 3 Lysate (NBL1-15730). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Secretogranin 3 Lysate (NBL1-15730) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Secretogranin 3 Products

Bioinformatics Tool for Secretogranin 3 Lysate (NBL1-15730)

Discover related pathways, diseases and genes to Secretogranin 3 Lysate (NBL1-15730). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Secretogranin 3 Lysate (NBL1-15730)

Discover more about diseases related to Secretogranin 3 Lysate (NBL1-15730).
 

Pathways for Secretogranin 3 Lysate (NBL1-15730)

View related products by pathway.

PTMs for Secretogranin 3 Lysate (NBL1-15730)

Learn more about PTMs related to Secretogranin 3 Lysate (NBL1-15730).

Blogs on Secretogranin 3

There are no specific blogs for Secretogranin 3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Secretogranin 3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SCG3