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SEC24D Antibody (1A8)

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 (1 Review)
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Western Blot: SEC24D Antibody (1A8) [H00009871-M04] - Analysis of SEC24D expression in HeLa (Cat # L013V1).
Western Blot: SEC24D Antibody (1A8) [H00009871-M04] - Analysis of SEC24D expression in HeLa (Cat # L013V1).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SEC24D Antibody (1A8) Summary

Immunogen
SEC24D (NP_055637, 935 a.a. ~ 1033 a.a) partial recombinant protein with GST tag.SPPELIQGIFNVPSFAHINTDMTLLPEVGNPYSQQLRMIMGIIQQKRPYSMKLTIVKQREQPEMVFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN
Specificity
SEC24D - SEC24 related gene family, member D (S. cerevisiae)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SEC24D
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Reviewed Applications
Read 1 Review rated 3
using
H00009871-M04 in the following applications:
Publications
Read Publications using H00009871-M04.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SEC24D Antibody (1A8)

  • FLJ43974
  • KIAA0755member D
  • SEC24 family, member D (S. cerevisiae)
  • SEC24 related gene family, member D

Background

The protein encoded by this gene is a member of the SEC24 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. This gene product is implicated in the shaping of the vesicle, and also in cargo selection and concentration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: SEC24B Antibody [NBP1-86263] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SEC24B Antibody [NBP1-86263] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
SEC24C Antibody
NBP1-81550
Western Blot: SEC24C Antibody [NBP1-81550] - Analysis in human cell lines PC-3 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/Immunofluorescence: SEC24C Antibody [NBP1-81550] - Staining of human cell line U-251 MG shows localization to nucleoplasm and vesicles.
Immunohistochemistry-Paraffin: SEC24C Antibody [NBP1-81550] - Staining of human stomach, lower shows moderate granular cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
GAT-1/SLC6A1
Antibody
NBP1-89802
Western Blot: GAT-1/SLC6A1 Antibody [NBP1-89802] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Staining in human cerebral cortex and skeletal muscle tissues. Corresponding SLC6A1 RNA-seq data are presented for the same tissues.
Western Blot: GAT-1/SLC6A1 Antibody [NBP1-89802] - Analysis in human liver tissue.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
SEC24A Antibody
NBP2-58626
Western Blot: SEC24A Antibody [NBP2-58626] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/Immunofluorescence: SEC24A Antibody [NBP2-58626] - Staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & vesicles.
Species: Hu
Applications: WB, ICC/IF

SLC6A4/5-HTTLPR/Seroto ...
H00006532-D01P
Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Analysis of SLC6A4 expression in mouse lung.
Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Analysis of SLC6A4 expression in transfected 293T cell line by SLC6A4 polyclonal antibody.Lane 1: SLC6A4 transfected lysate(70.30 KDa).Lane 2: Non-transfected lysate.
(1 Publication)
Species: Hu, Mu
Applications: WB
SEC23A Antibody
NBP2-34842
Western Blot: SEC23A Antibody [NBP2-34842] - Analysis was performed whole cell extracts and tissue extracts of HeLa (Lane 1), PC-12 (Lane 2), NIH/3T3 (Lane 3), Mouse Liver (Lane 4) and Rat Liver (Lane 5).
Immunocytochemistry/Immunofluorescence: SEC23A Antibody [NBP2-34842] - Analysis of COP II (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: SEC23A Antibody [NBP2-34842] - Analysis of COP II was done on PC-12 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with COP II Rabbit Polyclonal Antibody (PA1-069A, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA.
Species: Hu, Mu, Rt, Ha
Applications: WB, Flow, ICC/IF, IHC, IP
SEC23B Antibody
NBP1-85732
Immunocytochemistry/Immunofluorescence: SEC23B Antibody [NBP1-85732] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles. Antibody staining shown in green.
Immunohistochemistry-Paraffin: SEC23B Antibody [NBP1-85732] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells and moderate staining in Islet cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Syntaxin-BP1
Antibody
NBP1-83139
Western Blot: Syntaxin-BP1 Antibody [NBP1-83139] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: Syntaxin-BP1 Antibody [NBP1-83139] - Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody [NBP1-83139] - Staining in human cerebral cortex and pancreas tissues using anti-STXBP1 antibody. Corresponding STXBP1 RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
GPR151 Antibody
NLS418
Immunohistochemistry-Paraffin: GPR151 Antibody [NLS418] - Anti-GPR151 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: GPR151 Antibody [NLS418] - Analysis of anti-GPR151 antibody with brain, thalamus at 2 ug/ ml.
Immunohistochemistry-Paraffin: GPR151 Antibody [NLS418] - Analysis of anti-GPR151 antibody with brain, neurons.
Species: Hu
Applications: IHC-P
AKR1C2 Antibody
(3C11)
H00001646-M03
Western Blot: AKR1C2 Antibody (3C11) [H00001646-M03] - Analysis of AKR1C2 expression in transfected 293T cell line by AKR1C2 monoclonal antibody (M03), clone 3C11. Lane 1: AKR1C2 transfected lysatE (36.7 KDa). Lane 2: Non-transfected lysate.
ELISA: AKR1C2 Antibody (3C11) [H00001646-M03] - Detection limit for recombinant GST tagged AKR1C2 is approximately 0.3ng/ml as a capture antibody.
(2 Publications)
Species: Hu
Applications: WB, ELISA
SAR1 Antibody
NBP1-80789
Western Blot: SAR1 Antibody [NBP1-80789] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SAR1A antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: SAR1 Antibody [NBP1-80789] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Western Blot: SAR1 Antibody [NBP1-80789] - Analysis in human cell line A-431.
Species: Hu
Applications: WB, IHC, IHC-P, KD
Carboxypeptidase
E/CPE Antibody [Uncon ...
AF3587
A172 human glioblastoma cell line was stained with Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3587, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol and saponin.
Carboxypeptidase E/CPE was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Carboxypeptidase E/CPE was detected in immersion fixed frozen sections of mouse embryo (9.5 d.p.c.) using Goat Anti-Human Carboxypeptidase E/CPE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3587) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the developing liver. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Species: Hu
Applications: WB, IHC, IP, CyTOF-ready, ICC, ICFlow
ARFGAP3 Antibody
NBP1-88842
Western Blot: ARFGAP3 Antibody [NBP1-88842] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: ARFGAP3 Antibody [NBP1-88842] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: ARFGAP3 Antibody [NBP1-88842] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NeuroD1 Antibody
(3H8)
H00004760-M01
Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.
(1 Publication)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
IQGAP1 Antibody
NBP1-06529
Western Blot: IQGAP1 Antibody [NBP1-06529] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1- 06529 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-06529] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-IQGAP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Proximity Ligation Assay: IQGAP1 Antibody [NBP1-06529] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MEN1 (NB100-287) and rabbit anti-human IQGAP1 (NBP1-06529). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X mangification.
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, IHC-P, IP, PLA
ARFGAP1 Antibody
(OTI1F6)
NBP2-00513
Western Blot: ARFGAP1 Antibody (1F6) [NBP2-00513] Analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARFGAP1.
Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human thyroid tissue using anti-ARFGAP1 mouse monoclonal antibody.
Species: Hu, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
SLC6A12 Antibody
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Western Blot: SLC6A12 Antibody [NBP1-88641] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: SLC6A12 Antibody [NBP1-88641] - Staining of human kidney shows moderate cytoplasmic and luminal membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: SLC6A12 Antibody [NBP1-88641] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Species: Hu
Applications: WB, IHC, IHC-P
SEC24D Antibody
(1A8)
H00009871-M04
SEC24D Antibody (1A8)
Species: Hu
Applications: WB, ELISA

Publications for SEC24D Antibody (H00009871-M04)(3)


Review for SEC24D Antibody (H00009871-M04) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00009871-M04:
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Western Blot SEC24D H00009871-M04
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reviewed by:
Anonymous
 WB Human 02/10/2016
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Summary

ApplicationWestern Blot
SpeciesHuman
LotH00009871-M04
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Western Blot SEC24D H00009871-M04
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 Anonymous
02/10/2016
Application: WB
Species: Human
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Bioinformatics

Gene Symbol SEC24D
Entrez
OMIM
Uniprot