SDHA Antibody

Western Blot: SDHA Antibody [NBP2-99321] - Anti-SDHA rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 72 kDa

Immunocytochemistry/Immunofluorescence: SDHA Antibody [NBP2-99321] - Immunofluorescence staining of SDHA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SDHA polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.

Immunohistochemistry-Paraffin: SDHA Antibody [NBP2-99321] - Immunochemical staining of human SDHA in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: SDHA Antibody [NBP2-99321] - Immunochemical staining of human SDHA in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: SDHA Antibody [NBP2-99321] - SDHA was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate Lane B: 0.5 mg HeLa Whole Cell Lysate Lane C: 0.5 mg Jurkat Whole Cell Lysate 2 ul anti-SDHA rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SDHA rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 72 kDa

Product Discontinued

SDHA Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SDHA.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SDHA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for SDHA Antibody

• CMD1GG
• flavoprotein subunit of complex II
• FP
• MC2DN1
• NDAXOA
• PGL5
• PPGL5
• SDH1
• SDH2
• SDHA
• SDHF
• succinate dehydrogenase complex, subunit A, flavoprotein (Fp)

Background

The SDHA gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDHA