SCIMP Antibody (NVL-07) - BSA Free

Flow Cytometry: SCIMP Antibody (NVL-07) [NBP2-22349] - Intracellular staining of SCIMP in a population of HEK-293T-SCIMP transfectants using monoclonal antibody (clone NVL-07, purified).The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IP, CyTOF-ready
• Clone: NVL-07
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SCIMP Antibody (NVL-07) - BSA Free Summary

• Immunogen: Recombinant intracellular part of human SCIMP.
• Specificity: The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCIMP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Purity: > 95% by SDS-PAGE, Flow Cytometry - intracellular staining. This antibody is CyTOF ready.
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for SCIMP Antibody (NVL-07) - BSA Free

• transmembrane protein C17orf87
• C17orf87
• chromosome 17 open reading frame 87
• DTFT5783
• FLJ32580
• SLP adapter and CSK-interacting membrane protein
• SLP adaptor and CSK interacting membrane protein
• SLP65/SLP76, Csk-interacting membrane protein
• UNQ5783

Background

SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCIMP
• Uniprot:
  • Q6UWF3(Human)