SALM3/LRFN4 Antibody (578102)

SALM3/LRFN4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human SALM3/LRFN4 Monoclonal Antibody (Catalog # MAB5445) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Product Discontinued

SALM3/LRFN4 Antibody (578102) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human SALM3/LRFN4
  Cys17-Leu518
  Accession # Q6PJG9
• Specificity: Detects human SALM3/LRFN4 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) SALM1, approximately 20-25% cross-reactivity with recombinant human rhSALM2, and no cross-reactivity with rhSALM4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LRFN4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SALM3/LRFN4 Antibody (578102)

• FIGLER6
• LRFN4
• SALM3
• SALM3.

Background

Synaptic adhesion-like molecule 3 (SALM3; also leucine-rich repeat and fibronectin type-III domain-containing protein 4 (LRFN4) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1).  Human SALM3 is synthesized as a 635 amino acid (aa) precursor that contains a 16 aa signal sequence, a 502 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 96 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2-like domain, and a fibronectin type-III domain, tandemly aligned in that order (1 - 2).  In addition, there are six potential sites for N-linked glycosylation.  The C-terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1 - 3, but is absent in SALMs 4 and 5 (3). Mature human SALM3 shares 97% aa sequence identity with mature mouse SALM3.  Northern blot analysis showed that in mice, SALM3 is strongly expressed in the adult brain and is also present in the adult testis (1). It is distributed throughout the neuron, including the growth cone (3).  In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM3, like the other SALMs, promotes neurite outgrowth (3).  Specifically, the SALMs modify total outgrowth and neurite branching (3).  SALM3 may also be involved in synapse formation, synaptic maintenance, and other cellular interactions (3).

1. Morimura, N. et al. (2006) Gene 380:72.
2. Wang, C.Y. et al. (2006) J. Neurosci. 26:2174.
3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SALM3/LRFN4 Antibody (MAB5445)(1)

Publication using MAB5445

• Y Liu, X Chen, X Chen, X Yang, Q Song, H Wu High SALM3 Expression in Tumor Cells and Fibroblasts Is Correlated with Poor Prognosis in Gastric Cancer Patients Dis. Markers, 2019-04-04;2019(0):8282414. 2019-04-04 [PMID: 31089399] (IHC-P, Human)

Bioinformatics

• Gene Symbol: LRFN4
• Uniprot:
  • Q6PJG9(Human)