Ryanodine Receptor 2 Antibody (C3-33) [Alexa Fluor™ Plus 488]

• Reactivity: Hu, Mu, Rt, Po, Am, Bv, Ca, Ch, Fi, Gp, Rb
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: C3-33
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 488

Ryanodine Receptor 2 Antibody (C3-33) [Alexa Fluor™ Plus 488] Summary

• Immunogen: Canine cardiac ryanodine receptor (RyR2)
• Localization: Membrane; Multi-pass membrane protein
• Specificity: Detects Ryanodine Receptor 2. Also shows cross-reactivity with Ryanodine Receptor 1 in amphibian, canine, chicken, fish, guinea pig and rat tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RYR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Ryanodine Receptor 2 Antibody (C3-33) [Alexa Fluor™ Plus 488]

• arrhythmogenic right ventricular dysplasia 2
• ARVC2
• ARVD2
• cardiac muscle ryanodine receptor-calcium release channel
• Cardiac muscle-type ryanodine receptor
• cardiac-type ryanodine receptor
• hRYR-2
• islet-type ryanodine receptor
• kidney-type ryanodine receptor
• ryanodine receptor 2 (cardiac)
• Ryanodine Receptor 2
• RyR2
• RYR-2
• type 2 ryanodine receptor
• VTSIP

Background

Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The alpha1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR alpha1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR alpha1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RYR2