Ryanodine Receptor 1 Antibody

Immunohistochemistry: Ryanodine Receptor 1 Antibody [NBP2-76960] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RYR1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Ryanodine Receptor 1 Antibody [NBP2-76960] - Flow cytometric analysis of SiHa cells with RYR1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: Ryanodine Receptor 1 Antibody [NBP2-76960] - Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RYR1 antibody. Counter stained with hematoxylin.

Dot Blot: Ryanodine Receptor 1 Antibody [NBP2-76960] - Dot blot analysis of anti-RYR1 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR1 antibody was diluted with 1/500.

Product Discontinued

Ryanodine Receptor 1 Antibody Summary

• Immunogen: Synthetic peptide within Human Ryanodine Receptor 1 aa 1,354-1,403 / 5,038. (SwissProt: P21817 Human; SwissProt: E9PZQ0 Mouse)
• Localization: Sarcoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RYR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:500-1:1000
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
• Theoretical MW: 565 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ryanodine Receptor 1 Antibody

• CCO
• central core disease of muscle
• MHS
• MHS1
• PPP1R137
• protein phosphatase 1, regulatory subunit 137
• ryanodine receptor 1 (skeletal)
• Ryanodine Receptor 1
• ryanodine receptor type1
• RYDR
• RYR
• RyR1
• RYR-1
• sarcoplasmic reticulum calcium release channel
• Skeletal muscle calcium release channel
• skeletal muscle ryanodine receptor
• Skeletal muscle-type ryanodine receptor
• SKRR
• type 1-like ryanodine receptor

Background

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RYR1