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RTN1-A/NSP Antibody (RNL-2) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Pm, RbSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
RNL-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-97736AF350
Alexa Fluor 405
NBP1-97736AF405
Alexa Fluor 488
NBP1-97736AF488
Alexa Fluor 532
NBP1-97736AF532
Alexa Fluor 594
NBP1-97736AF594
Alexa Fluor 647
NBP1-97736AF647
Alexa Fluor 700
NBP1-97736AF700
Alexa Fluor 750
NBP1-97736AF750
Allophycocyanin
NBP1-97736APC
Allophycocyanin/Cy7
NBP1-97736APCCY7
Biotin
NBP1-97736B
DyLight 350
NBP1-97736UV
DyLight 405
NBP1-97736V
DyLight 488
NBP1-97736G
DyLight 550
NBP1-97736R
DyLight 594
NBP1-97736DL594
DyLight 650
NBP1-97736C
DyLight 680
NBP1-97736FR
DyLight 755
NBP1-97736IR
FITC
NBP1-97736F
Janelia Fluor 549
NBP1-97736JF549
PE
NBP1-97736PE
PE/Atto594
NBP1-97736PEATT594
PE/Cy5.5
NBP1-97736PECY55
PE/Cy7
NBP1-97736PECY7
PerCP
NBP1-97736PCP
Unconjugated
NBP1-97736
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RTN1-A/NSP Antibody (RNL-2) [Janelia Fluor® 646] Summary

Immunogen
RNL-2 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with the small cell lung cancer cell line NCI-H82. RNL-2 recognizes an epitope loCated within the region of amino acids 421-589 of the neuro-endocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B).
Specificity
In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RTN1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Reacts with Rhesus Primate.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for RTN1-A/NSP Antibody (RNL-2) [Janelia Fluor® 646]

  • NSP-A
  • RTN1
  • RTN1A
  • RTN1-A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TFF2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human TFF2 Monoclonal Antibody (Catalog # MAB4077) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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DMPK Antibody
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Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human DMPK Monoclonal Antibody (Catalog # MAB6967) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DMPK at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Nogo Antibody
NB100-56681
Western Blot: Nogo Antibody [NB100-56681] - Analysis of Nogo A and B in A) human, B) mouse, and C) rat brain tissue lysate using Nogo antibody at 1:2000.
Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681] - Analysis of human testis using Nogo antibody at 1:100.
Simple Western: Nogo Antibody [NB100-56681] - Lane view shows a specific band for Nogo in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(16 Publications)
Species: Hu, Mu, Rt, Rb, Sh
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P
RTN3 Antibody
NBP1-88871
Immunocytochemistry/Immunofluorescence: RTN3 Antibody [NBP1-88871] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human cerebral cortex, colon, liver and lymph node using Anti-RTN3 antibody (A) NBP1-88871 shows similar protein distribution across tissues to independent antibody NBP1-88870 (B).
Immunohistochemistry-Paraffin: RTN3 Antibody [NBP1-88871] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Filamin A Antibody
(4E10-1B2)
H00002316-M01
Western Blot: Filamin A Antibody (4E10-1B2) [H00002316-M01] - FLNA monoclonal antibody (M01), clone 4E10-1B2 Analysis of FLNA expression in HL-60.
Immunohistochemistry-Paraffin: Filamin A Antibody (4E10-1B2) [H00002316-M01] - Analysis of monoclonal antibody to FLNA on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1.5 ug/ml.
Proximity Ligation Assay: Filamin A Antibody (4E10-1B2) [H00002316-M01] - Analysis of protein-protein interactions between GP1BA and FLNA. HeLa cells were stained with anti-GP1BA rabbit purified polyclonal 1:1200 and anti-FLNA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
(11 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, PLA, S-ELISA
Human
Ceruloplasmin ELISA K ...
NBP2-60616
ELISA: Human Ceruloplasmin ELISA Kit (Colorimetric) [NBP2-60616]
Species: Hu
Reticulon 2
Antibody
NBP1-59662
Western Blot: Reticulon 2 Antibody [NBP1-59662] - Jurkat cell lysate, concentration 2.5 ug/ml.
Immunohistochemistry-Paraffin: Reticulon 2 Antibody [NBP1-59662] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.
Species: Hu
Applications: WB, IHC, IHC-P
MMP-12 Antibody
NBP1-31225
Western Blot: MMP-12 Antibody [NBP1-31225] - Sample(30 ug whole cell lysate) A:293T 10% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: MMP-12 Antibody [NBP1-31225] - Analysis of methanol-fixed H1299, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MMP-12 Antibody [NBP1-31225] - Imaging of Human breast cancer tissue. MMP12 is stained by antibody (green) and nucleus is stained with DAPI (blue). This image was submitted via customer Review.
(2 Reviews)
(3 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
KRAS Antibody
(3B10-2F2)
H00003845-M01
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of monoclonal antibody to KRAS on HeLa cell. Antibody concentration 10 ug/ml.
(1 Review)
(13 Publications)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IP, S-ELISA
HSD11B2 Antibody
NBP2-37898
Western Blot: HSD11B2 Antibody [NBP2-37898] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Kidney tissue
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human rectum.
Immunohistochemistry-Paraffin: HSD11B2 Antibody [NBP2-37898] - Staining of human kidney shows high expression.
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Applications: WB, IHC, IHC-P
REEP5 Antibody
(OTI4D2) - Azide and ...
NBP2-73850
Western Blot: REEP5 Antibody (OTI4D2) - Azide and BSA Free [NBP2-73850] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY REEP5.
Immunohistochemistry: REEP5 Antibody (OTI4D2) - Azide and BSA Free [NBP2-73850] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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p130Cas/BCAR1
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NBP2-00532
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Immunocytochemistry/Immunofluorescence: BCAR1 Antibody (1A9) [NBP2-00532] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1.
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RTN1-A/NSP
Antibody (RNL-2) [Jan ...
NBP1-97736JF646
RTN1-A/NSP Antibody (RNL-2) [Janelia Fluor® 646]
Species: Hu, Pm, Rb
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-97736AF350
Alexa Fluor 405 NBP1-97736AF405
Alexa Fluor 488 NBP1-97736AF488
Alexa Fluor 532 NBP1-97736AF532
Alexa Fluor 594 NBP1-97736AF594
Alexa Fluor 647 NBP1-97736AF647
Alexa Fluor 700 NBP1-97736AF700
Alexa Fluor 750 NBP1-97736AF750
Allophycocyanin NBP1-97736APC
Allophycocyanin/Cy7 NBP1-97736APCCY7
Biotin NBP1-97736B
DyLight 350 NBP1-97736UV
DyLight 405 NBP1-97736V
DyLight 488 NBP1-97736G
DyLight 550 NBP1-97736R
DyLight 594 NBP1-97736DL594
DyLight 650 NBP1-97736C
DyLight 680 NBP1-97736FR
DyLight 755 NBP1-97736IR
FITC NBP1-97736F
Janelia Fluor 549 NBP1-97736JF549
Janelia Fluor 646 NBP1-97736JF646
PE NBP1-97736PE
PE/Atto594 NBP1-97736PEATT594
PE/Cy5.5 NBP1-97736PECY55
PE/Cy7 NBP1-97736PECY7
PerCP NBP1-97736PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol RTN1