Recombinant Human RSK3 Protein

SDS-Page: Recombinant RSK3 Protein [NBP1-99503]

Product Discontinued

Recombinant Human RSK3 Protein Summary

• Description: Recombinant protein for Human RSK3
  
  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NM_021135)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab60882)
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: RPS6KA2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze one pmol Suc-LLVY-AMC per minute at 25 C.
  Specific activity: The specific activity of RSK3 was determined to be 131 nmol /min/mg as per activity assay protocol.
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human RSK3 Protein

• EC 2.7.11
• EC 2.7.11.1
• HU-2
• MAP kinase-activated protein kinase 1c
• MAPK-activated protein kinase 1c
• MAPKAP kinase 1c
• MAPKAPK1C
• MAPKAPK-1c
• p90-RSK 2
• p90RSK2
• p90-RSK3
• ribosomal protein S6 kinase alpha 2
• ribosomal protein S6 kinase alpha-2
• ribosomal protein S6 kinase, 90kD, polypeptide 2
• ribosomal protein S6 kinase, 90kDa, polypeptide 2
• Ribosomal S6 kinase 3,90 kDa ribosomal protein S6 kinase 2
• RPS6KA2
• RSK
• RSK3
• RSK-3
• RSK3pp90RSK3
• S6K-alpha
• S6K-alpha2
• S6K-alpha-2

Background

RSK3 is a member of the RSK (ribosomal S6 kinase) family that encodes a 733-amino-acid protein with a unique N-terminal region containing a putative nuclear localization signal (1). RSK3 mRNA is widely expressed and is activated by growth factors, serum and phorbol ester. Upon stimulation, RSK3 translocates to the cell nucleus and phosphorylates nuclear proteins. RSK3 can bind to ERK1/2 and this association increases the duration of RSK3 activation (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA2