RPL9 Antibody (2F11)

Western Blot: RPL9 Antibody (2F11) [H00006133-M01] - RPL9 monoclonal antibody (M01), clone 2F11 Analysis of RPL9 expression in HeLa.

Immunocytochemistry/Immunofluorescence: RPL9 Antibody (2F11) [H00006133-M01] - Analysis of monoclonal antibody to RPL9 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: RPL9 Antibody (2F11) [H00006133-M01] - Analysis of monoclonal antibody to RPL9 on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 1 ~ 10 ug/ml.

Sandwich ELISA: RPL9 Antibody (2F11) [H00006133-M01] - Detection limit for recombinant GST tagged RPL9 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
• Clone: 2F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RPL9 Antibody (2F11) Summary

• Immunogen: RPL9 (NP_000652, 93 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RSVYAHFPINVVIQENGSLVEIRNFLGEKYIRRVRMRPGVACSVSQAQKDELILEGNDIELVSNSAALIQQATTVKNKDIRKFLDGIYVSEKGTVQQAD
• Specificity: RPL9 - ribosomal protein L9
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPL9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RPL9 Antibody (2F11)

• DKFZp313J1510
• FLJ27456
• MGC15545
• NPC-A-16
• ribosomal protein L9,60S ribosomal protein L9
• RPL9P7
• RPL9P8
• RPL9P9

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RPL9 Antibody (H00006133-M01)(3)

Publications using H00006133-M01

• Kanda S, Yanagitani K, Yokota Y et al. Autonomous translational pausing is required for XBP1u mRNA recruitment to the ER via the SRP pathway. Proc Natl Acad Sci U S A 2016-09-20 [PMID: 27651490]
• de Mateo S, Castillo J, Estanyol JM et al. Proteomic characterization of the human sperm nucleus. Proteomics 2011-06-02 [PMID: 21630459]
• de Mateo S, Castillo J, Estanyol JM et al. Proteomic characterization of the human sperm nucleus. PROTEOMICS 2011-06-01 [PMID: 21080497]

Bioinformatics

• Gene Symbol: RPL9
• Entrez:
  • 6133(Human)
• OMIM:
  • 603686(Human)
• Uniprot:
  • NP_000652(Human)