Recombinant Human ROBLD3 His Protein

SDS-Page: Recombinant Human ROBLD3 Protein [NBP1-50918] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human ROBLD3 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-125 of Human ROBLD3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLRPKA LTQVLSQANT GGVQSTLLLN NEGSLLAYSG YGDTDARVTA AIASNIWAAY DRNGNQAFNE DNLKFILMDC MEGRVAITRV ANLLLCMYAK ETVGFGMLKA KAQALVQYLE EPLTQVAAS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: LAMTOR2
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.2 M Nacl, 2 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human ROBLD3 His Protein

• ENDAP
• Endosomal adaptor protein p14
• HSPC003
• Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2
• late endosomal/lysosomal adaptor, MAPK and MTOR activator 2
• Late endosomal/lysosomal Mp1-interacting protein
• MAPBPIPRoadblock domain-containing protein 3
• MAPKSP1 adaptor protein
• MAPKSP1AP
• mitogen-activated protein-binding protein-interacting protein (MAPBPIP)
• Mitogen-activated protein-binding protein-interacting protein
• p14Ragulator2
• roadblock domain containing 3
• ROBLD3

Background

ROBLD3, also known as MAPBPIP, belongs to the GAMAD family. It is an adapter protein that enhances the efficiency of the MAP kinase cascade and facilitates the activation of MAPK2. ROBLD3 compels the recruitment of MP1 to late endosomes where they form a very stable heterodimeric complex required for ERK activation on endosomes. Recombinant human ROBLD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: LAMTOR2