ROBLD3 Overexpression Lysate (Native)

Images

 
Western Blot: ROBLD3 Overexpression Lysate (Adult Normal) [NBL1-12864] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ROBLD3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ROBLD3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200862 and based on accession number NM_014017. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens roadblock domain containing 3 (ROBLD3), transcript variant 1, mRNA.
Gene
LAMTOR2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
13.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ROBLD3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ROBLD3 Overexpression Lysate (Native)

  • ENDAP
  • Endosomal adaptor protein p14
  • HSPC003
  • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2
  • late endosomal/lysosomal adaptor, MAPK and MTOR activator 2
  • Late endosomal/lysosomal Mp1-interacting protein
  • MAPBPIPRoadblock domain-containing protein 3
  • MAPKSP1 adaptor protein
  • MAPKSP1AP
  • mitogen-activated protein-binding protein-interacting protein (MAPBPIP)
  • Mitogen-activated protein-binding protein-interacting protein
  • p14Ragulator2
  • roadblock domain containing 3
  • ROBLD3

Background

The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LAMTOR2