Rhodopsin Antibody (4D2)

Product Discontinued

Rhodopsin Antibody (4D2) Summary

• Immunogen: N-terminus of Rhodopsin(NP_001014890.1)
• Localization: Membrane
• Specificity: Detects approx 40kDa. Binds specifically to the N-terminus of Rhodopsin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHO
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:1000

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30052645). Based on homology, It is predicted to detect Mammals , Fish , Anchovies , Avian , Amphibians , Shark and Extinct Spiny Shark (Acanthodes bridgei). Use in Mouse reported in secitific publication PMID: 32460314

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Rhodopsin Antibody (4D2)

• CSNBAD1
• MGC138309
• OPN2
• OPN2MGC138311
• opsin 2, rod pigment
• Opsin-2
• retinitis pigmentosa 4, autosomal dominant
• RHO
• Rhodopsin
• RP4

Background

Rhodopsin is the first member identified in the Opsin subfamily of GPCRs. Rhodopsin mediates dim-light vision by maximally absorbing light at 495 nm. Mutations in rhodopsin cause retinitis pigmentosa, an inherited disease that initially causes night blindness and loss of the mid-peripheral visual field, and eventually leads to a total loss of vision. Rhodopsin expression has been reported in the rod-shaped photoreceptor cells of the retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rhodopsin Antibody (NBP1-48334)(8)

Publications using NBP1-48334

• Wang T, Yao J, Jia L et al. Loss of alpha-A or alpha B-Crystallin Accelerates Photoreceptor Cell Death in a Mouse Model of P23H Autosomal Dominant Retinitis Pigmentosa International Journal of Molecular Sciences 2021-12-22 [PMID: 35008496] (WB, IF/IHC, Mouse)
• Ross BX, Choi J, Yao J et al. Loss of High-Mobility Group Box 1 (HMGB1) Protein in Rods Accelerates Rod Photoreceptor Degeneration After Retinal Detachment Invest. Ophthalmol. Vis. Sci. 2020-05-11 [PMID: 32460314] (IHC-P, Mouse)
• Qiu Y, Yao J, Jia L et al. Shifting the balance of autophagy and proteasome activation reduces proteotoxic cell death: a novel therapeutic approach for restoring photoreceptor homeostasis Cell Death Dis 2019-07-18 [PMID: 31320609] (WB, Mouse)
• Messchaert M, Dona M, Broekman S et al. Eyes shut homolog is important for the maintenance of photoreceptor morphology and visual function in zebrafish PLoS ONE 2018-07-27 [PMID: 30052645] (IHC-Fr, Zebrafish)
• Corral-Serrano JC, Messchaert M, Dona M et al. C2orf71a/pcare1 is important for photoreceptor outer segment morphogenesis and visual function in zebrafish. Sci Rep. 2018-06-26 [PMID: 29946172] (IF/IHC, Zebrafish)
• Yao J, Qiu Y, Frontera E et al. Inhibiting autophagy reduces retinal degeneration caused by protein misfolding. Autophagy. 2018-07-13 [PMID: 29940785] (WB, Mouse)
• Siuda ER, McCall JG, Al-Hasani R et al. Optodynamic simulation of beta-adrenergic receptor signalling. Nat Commun 2015-09-28 [PMID: 26412387] (WB)
• Grison A, Zucchelli S, Urzi A et al. Mesencephalic dopaminergic neurons express a repertoire of olfactory receptors and respond to odorant-like molecules. BMC Genomics. 2014-08-27 [PMID: 25164183] (WB, Human)

Bioinformatics

• Gene Symbol: RHO
• Uniprot:
  • P08100(Human)