Rhodopsin Antibody (4D2)

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Immunocytochemistry/ Immunofluorescence: Rhodopsin Antibody (4D2) [NBP1-48334]

Product Details

Summary
Product Discontinued
View other related Rhodopsin Primary Antibodies

Order Details


    • Catalog Number
      NBP1-48334
    • Availability
      Product Discontinued

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Rhodopsin Antibody (4D2) Summary

Immunogen
N-terminus of Rhodopsin(NP_001014890.1)
Localization
Membrane
Specificity
Detects approx 40kDa. Binds specifically to the N-terminus of Rhodopsin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RHO
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of Rhodopsin Antibody was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Publications
Read Publications using
NBP1-48334 in the following applications:

  • WB
    2 publications

Reactivity Notes

Based on homology, It is predicted to detect Mammals, Fish, Anchovies, Avian, Amphibians, Shark and Extinct Spiny Shark (Acanthodes bridgei)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Rhodopsin Antibody (4D2)

  • CSNBAD1
  • MGC138309
  • OPN2MGC138311
  • opsin 2, rod pigment
  • opsin-2
  • retinitis pigmentosa 4, autosomal dominant
  • rhodopsin
  • RP4

Background

Rhodopsin is the first member identified in the Opsin subfamily of GPCRs. Rhodopsin mediates dim-light vision by maximally absorbing light at 495 nm. Mutations in rhodopsin cause retinitis pigmentosa, an inherited disease that initially causes night blindness and loss of the mid-peripheral visual field, and eventually leads to a total loss of vision. Rhodopsin expression has been reported in the rod-shaped photoreceptor cells of the retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rhodopsin Antibody (NBP1-48334)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Rhodopsin Products

Bioinformatics Tool for Rhodopsin Antibody (NBP1-48334)

Discover related pathways, diseases and genes to Rhodopsin Antibody (NBP1-48334). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Rhodopsin Antibody (NBP1-48334)

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Pathways for Rhodopsin Antibody (NBP1-48334)

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PTMs for Rhodopsin Antibody (NBP1-48334)

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Research Areas for Rhodopsin Antibody (NBP1-48334)

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Blogs on Rhodopsin.

Vision Infographic: Do you see how I see?
Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

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Bioinformatics

Gene Symbol RHO
Uniprot