RHBDF2 Antibody (996308) [Unconjugated]

Western blot shows lysates of LoVo human colorectal adenocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human iRHOM2/RHBDF2 Monoclonal Antibody (Catalog # MAB10048) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iRHOM2/RHBDF2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 996308
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RHBDF2 Antibody (996308) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human iRHOM2/RHBDF2
  Accession # Q6PJF2
• Specificity: Detects huma iRHOM2/RHBDF2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RHBDF2 Antibody (996308) [Unconjugated]

• FLJ22341
• iRHOM2
• RHBDF2
• RHBDL5
• RHBDL5rhomboid family member 2
• RHBDL6
• rhomboid 5 homolog 2 (Drosophila)
• Rhomboid 5 homolog 2
• rhomboid veinlet-like 5
• Rhomboid veinlet-like protein 5
• Rhomboid veinlet-like protein 6
• rhomboid, veinlet-like 6 (Drosophila)
• rhomboid, veinlet-like 6
• rhomboid-like protein 6
• TOCG

Background

Human Inactive rhomboid protein 2 (RHBDF2 or IRHOM2) is a highly conserved seven-transmembrane Rhomboid protease-like protein which is inactive, it has no protease activity, but has been implicated in the secretion of several ligands of the epidermal growth factor receptor (EGFR), probably by regulating trafficking and stability of key protein membrane like ADAM17 and STING. The RHBDF2 protein plays an important role in the secretion of tumor necrosis factor alpha and has been implicated in familial esophageal cancer. Gain-of-function (GOF) mutations in the RHBDF2 gene constitutively activate EGFR signaling to cause tylosis with esophageal cancer syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RHBDF2 Antibody (MAB10048)(2)

Publications using MAB10048

• Klersy, A;Meyer, S;Leuschner, F;Kessler, T;Hecker, M;Wagner, AH; Ectodomain Shedding by ADAM17 Increases the Release of Soluble CD40 from Human Endothelial Cells under Pro-Inflammatory Conditions Cells 2023-07-25 [PMID: 37566005] (Western Blot, Human)
• AA Giese, A Babendreye, P Krappen, A Gross, P Strnad, S Düsterhöft, A Ludwig Inflammatory activation of surface molecule shedding by upregulation of the pseudoprotease iRhom2 in colon epithelial cells Scientific Reports, 2021-12-20;11(1):24230. 2021-12-20 [PMID: 34930929] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • Q6PJF2()