RHBDD3 Antibody - BSA Free

Western Blot: RHBDD3 Antibody [NBP1-76250] - Rat lung tissue lysate with RHBDD3 antibody at 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: RHBDD3 Antibody - BSA Free [NBP1-76250] - Immunofluorescence of RHBDD32 in rat lung tissue with RHBDD3 antibody at 20 u/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RHBDD3 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the center of human RHBDD3. The immunogen is located within amino acids 170 - 220 of RHBDD3. Amino Acid Squence: FRWLEPSERRLQV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RHBDD3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  RHBDD3 Peptide (NBP1-76250PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RHBDD3 Antibody - BSA Free

• C22orf3
• chromosome 22 open reading frame 3
• HS984G1A
• pituitary tumor apoptosis
• PTAG
• rhomboid domain containing 3
• rhomboid domain-containing protein 3

Background

The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD3 was initially identified as a novel pituitary tumor apoptosis gene (PTAG) whose expression was reduced in certain pituitary adenomas. Overexpression of RHBDD3 in AtT20 cells showed an increase in apoptotic activity and caspase activation in response to bromocriptine, and apoptosis-inducing dopamine D2 analog, suggesting that reactivation of RHBDD3 in tumors may be a therapeutically useful tool. Later experiments also showed that RHBDD3 expression is also reduced in several primary colorectal tumors, indicating that loss of RHBDD3 contributes to a blunted apoptotic response and probably predisposes cells towards malignant transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHBDD3
• Uniprot:
  • NP_036397(Human)