RGS18 Antibody

Product Discontinued

RGS18 Antibody Summary

• Immunogen: Peptide with sequence CNEFQDVQSDVAIWL corresponding to C-Terminus according to NP_570138.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RGS18
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 28 kDa band observed in human peripheral blood mononucleocytes (lymphocytes) lysates (calculated MW of 27.6 kDa band according to NP_570138.1).
• Control:

  RGS18 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RGS18 Antibody

• regulator of G-protein signaling 18
• regulator of G-protein signalling 13
• RGS13regulator of G-protein signalling 18

Background

Regulators of G-protein signaling (RGSs) are a protein family that can act as GTPase-activating proteins for G(alphai)- and G(alphaq)-class proteins. They accelerate transit through the cycle of GTP binding and hydrolysis and thereby accelerate signaling kinetics and termination. One of these proteins, RGS18, is a 235 amino acid protein that is closely related to RGS5 (46% identity) and approximately 30-40% identity to other RGS proteins. This protein is expressed in platelet, leukocyte, and megakaryocyte cell lines. Engagement of TLR3 or TLR4 on monocyte-derived DCs potently down-regulates RGS18 and RGS14 without modifying other RGS proteins. A similar pattern of RGS protein expression occurs in immature bone marrow-derived mouse DCs stimulated to mature via TLR4 signaling. The changes in RGS18 and RGS1 expression are likely important for DC function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RGS18 Antibody (NB300-807)(1)

Publication using NB300-807

• Park IK, Klug CA, Li K et al. Molecular cloning and characterization of a novel regulator of G-protein signaling from mouse hematopoietic stem cells. J Biol Chem 2001-01-12 [PMID: 11042171]

Bioinformatics

• Gene Symbol: RGS18
• Entrez:
  • 64407(Human)
• Uniprot:
  • NP_570138.(Human)