RGS18 Antibody - BSA Free

Western Blot: RGS18 Antibody [NB300-335] - E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-RGS18 )

Product Discontinued

RGS18 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide: INMCESKEKT FFKLIHGSGK EETSKEAKIR AKEKRNRLSL LVQKPEFHED TRSSRSGHLA KETRVSPEEA VKWGESFDKL LSHRDGLEAF, corresponding to amino acids 11-100 of RGS18.
• Epitope: aa 11-100
• Localization: Cytoplasmic
• Specificity: RGS18
• Clonality: Polyclonal
• Host: Chicken
• Gene: RGS18
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoaffinity Purification
  • Western Blot 1:200 - 1:1500
• Application Notes: This antibody can be used in Western Blot. Optimal dilutions should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for RGS18 Antibody - BSA Free

• regulator of G-protein signaling 18
• regulator of G-protein signalling 13
• RGS13regulator of G-protein signalling 18

Background

Regulators of G-protein signaling (RGSs) are a protein family that can act as GTPase-activating proteins for G(alphai)- and G(alphaq)-class proteins. They accelerate transit through the cycle of GTP binding and hydrolysis and thereby accelerate signaling kinetics and termination. One of these proteins, RGS18, is a 235 amino acid protein that is closely related to RGS5 (46% identity) and approximately 30-40% identity to other RGS proteins. This protein is expressed in platelet, leukocyte, and megakaryocyte cell lines. Engagement of TLR3 or TLR4 on monocyte-derived DCs potently down-regulates RGS18 and RGS14 without modifying other RGS proteins. A similar pattern of RGS protein expression occurs in immature bone marrow-derived mouse DCs stimulated to mature via TLR4 signaling. The changes in RGS18 and RGS1 expression are likely important for DC function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RGS18
• Entrez:
  • 64407(Human)
  • 64214(Mouse)
  • 289076(Rat)