RGS14 Antibody (N133/21) - Chimeric - Azide and BSA Free

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing cells stained NBP3-52342 (the BSA Free version of this antibody) using goat anti-chicken IgY secondary antibodyb(green). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the pyramidal neurons in the CA2 region of the hippocampus.

Western blotting of rat brain homogenate with RGS14 mouse monoclonal antibody (A) and NBP3-52342 (the BSA Free version of this antibody) (B). These antibodies were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. NBP3-52342 (the BSA Free version of this antibody) recognizes endogenous RGS14 with similar sensitivity as the RGS14 mouse monoclonal antibody in the rat brain homogenate at ~60 kDa

• Reactivity: Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: N133/21
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

RGS14 Antibody (N133/21) - Chimeric - Azide and BSA Free Summary

• Description: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N133/21 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N133/21 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 1-544 (full-length) of rat RGS14 (Uniprot#: O08773) produced recombinantly in E. Coli
• Specificity: Does not react with RGS12
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: RGS14
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry -Free Floating
  • Immunohistochemistry-Paraffin
  • Western Blot
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for RGS14 Antibody (N133/21) - Chimeric - Azide and BSA Free

• regulator of G-protein signaling 14
• regulator of G-protein signalling 14

Background

RGS14 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP bound form. Contains 1 GoLoco domain, 2 Ras binding (RBD) domains, 1 RGS domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RGS14