RFC3 Antibody (1C6) - Azide and BSA Free

Immunoprecipitation: RFC3 Antibody (1C6) [H00005983-M01] - Analysis of RFC3 transfected lysate using anti-RFC3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RFC3 monoclonal antibody.

Sandwich ELISA: RFC3 Antibody (1C6) [H00005983-M01] - Detection limit for recombinant GST tagged RFC3 is approximately 10ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 1C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RFC3 Antibody (1C6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RFC3 (AAH00149, 257 a.a. ~ 356 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETANAIVSQQTPQRLLEVRGRLYELLTHCIPPEIIMKGLLSELLHNCDGQLKGEVAQMAAYYEHRLQLGSKAIYHLEAFVAKFMALYKKFMEDGLEGMMF
• Specificity: RFC3 (1C6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RFC3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RFC3 Antibody (1C6) - Azide and BSA Free

• A1 38 kDa subunit
• Activator 1 38 kDa subunit
• Activator 1 subunit 3
• MGC5276
• replication factor C (activator 1) 3 (38kD)
• replication factor C (activator 1) 3, 38kDa
• Replication factor C 38 kDa subunit
• replication factor C subunit 3
• RFC, 38 kD subunit
• RFC38RF-C 38 kDa subunit

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFC3
• Entrez:
  • 5983(Human)
• OMIM:
  • 600405(Human)
• Uniprot:
  • AAH00149(Human)