RENT1/UPF1/hUPF1 Antibody

Product Discontinued

RENT1/UPF1/hUPF1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 900 and 950 of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: UPF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: By Western blot, this antibody recognizes a band at ~145 kDa, representing human RENT1. Suggested working dilutions: * Western Blot - 1:1,000-1:2,500 (ECL) Immunoprecipitation - ND Immunohistochemistry - ND *The investigator should determine the optimal working dilution for a specific application.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RENT1/UPF1/hUPF1 Antibody

• ATP-dependent helicase RENT1
• EC 3.6.1
• EC 3.6.4.-
• FLJ43809
• HUPF1
• KIAA0221FLJ46894
• Nonsense mRNA reducing factor 1
• NORF1
• NORF1delta helicase
• pNORF1
• regulator of nonsense transcripts 1
• RENT1
• RENT1UP Frameshift 1
• UPF1 regulator of nonsense transcripts homolog (yeast)
• UPF1
• up-frameshift mutation 1 homolog
• Up-frameshift suppressor 1 homolog
• yeast Upf1p homolog

Background

RENT1 (regulator of nonsense transcripts 1) is a group 1 RNA helicase, RNA-binding protein whose RNA-binding activity is modulated by ATP. It enters the nucleus where it influences early events in mRNA biogenesis. It functions in NMD (non-sense-mediated mRNA decay) in mammalian cells, ridding cells of aberrant mRNAs containing premature termination codons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RENT1/UPF1/hUPF1 Antibody (NB100-369)(1)

Publication using NB100-369

• Silva AL, Ribeiro P, Inacio A et al. Proximity of the poly(A)-binding protein to a premature termination codon inhibits mammalian nonsense-mediated mRNA decay. RNA 2008-03-01 [PMID: 18230761]

Bioinformatics

• Gene Symbol: UPF1
• Entrez:
  • 5976(Human)
• Uniprot:
  • NP_002902.2(Human)