Recombinant Rat Neurexophilin-3/NXPH3 Protein, CF

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Rat Neurexophilin-3/NXPH3 Protein, CF Summary

Details of Functionality
Measured in a competitive binding assay. When rrNeurexin-1 alpha (Catalog # 4485-NX) is immobilized at 1 µg/mL (100 µL/well), rrNeurexophilin-3 inhibits 50% binding of biotinylated rrNeurexophilin-1 (1 µg/mL) at the concentration range of 1‑3 µg/mL.
Source
Mouse myeloma cell line, NS0-derived rat Neurexophilin-3 protein
Gln23-Gly252, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln23 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
Nxph3
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
26.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-43 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Neurexophilin-3/NXPH3 Protein, CF

  • KIAA1159
  • neurexophilin 3
  • Neurexophilin3
  • Neurexophilin-3
  • NPH3
  • NPH3neurexophilin-3
  • NXPH3

Background

Neurexophilin-3 (NXPH3) is a neuropeptide-like secreted glycoprotein in the neurexophilin family (1, 2). The 252 amino acid (aa) NXPH3 precursor contains a 22 aa signal peptide, plus a 230 aa proprecursor that is likely cleaved at a basic motif, producing a 76 aa propeptide and a 154 aa mature protein (1). Mature rat NXPH3 shares 99%, 95% and 92% aa identity with mouse, human and bovine NXPH3, respectively. It contains motifs that are conserved among all neurexophilins, including three potential N-glycosylation sites in the N-terminal portion and six cysteines in the C-terminal portion (1). NXPH3 is expressed selectively in subplate-derived neurons in the cortex, granule cells in the vestibulocerebellum, and Cajal-Retzius cells during development (3). NXPH1 is the neurexophilin most similar to NXPH3, sharing 69% aa identity within the mature region. Expression of NXPH1 and NXPH3 does not appear to overlap, with NXPH1 expression occurring mainly by cells that resemble inhibitory interneurons (2 - 4). Both are tightly bound extracellular ligands of alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release (1, 5, 6). Genetic deletion of NXPH1 and/or NXPH3 produces no anatomical effect, although mice lacking NXPH3 show specific defects in motor coordination (3, 4). Of the other known neurexophilins, NXPH2 is not expressed in rodents, and NXPH4 does not bind alpha -neurexins (1, 5).

  1. Missler, M. and T. C. Sudhof (1998) J. Neurosci. 18:3630.
  2. Petrenko, A. G. et al. (1996) J. Neurosci. 16:4360.
  3. Beglopoulos, V. et al. (2005) Mol. Cell. Biol. 25:7278.
  4. Clarris, H. J. et al. (2002) Int. J. Dev. Biol. 46:649.
  5. Missler, M. et al. (1998) J. Biol. Chem. 273:34716.
  6. Dudanova, I. et al. (2006) J. Neurosci. 26:10599.

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Bioinformatics

Gene Symbol Nxph3
Uniprot