Recombinant Rat IL-1Rrp2/IL-1R6 Fc Chimera Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat IL-1Rrp2/IL-1R6 Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived rat IL-1 Rrp2/IL-1 R6 protein
  

  Rat IL-1 Rrp2 (Gly22 - Tyr340) Accession # Q62929	DIEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q62929
• N-terminal Sequence: Gly22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il1rl2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 63 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IL-1Rrp2/IL-1R6 Fc Chimera Protein, CF

• IL-1 R6
• IL-1 Rrp2
• IL1RL2
• IL-1RL2
• IL1Rrp2
• IL-1Rrp2
• IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2
• interleukin 1 receptor-like 2
• interleukin-1 receptor-like 2

Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll‑like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Rat IL-1 Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 319 aa extracellular domain. It shares 84% and 67% amino acid sequence identity with mouse and human IL‑1 Rrp2, respectively. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL-1 Rrp2 has been shown to mediate the activation of the transcription factor NF‑kB by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1e), with IL-1 RAcP as a co-factor (3, 4). Response to IL-1 F9 is specifically antagonized by IL-1 F5 (also known as IL-1d), an IL-1 family ligand that is most closely related to IL-1ra (3). IL-1 Rrp2, IL-1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses (3).

1. Boraschi, D. & A. Tagliabue (2006) Vitam. Horm. 74:229.
2. Lovenberg, T. W. et al. (1996) J. Neuroimmunol. 70:113.
3. Debets, R. et al. (2001) J. Immunol. 167:1440.
4. Towne, J. E. et al. (2004) J. Biol. Chem. 279:13677.

Bioinformatics

• Gene Symbol: Il1rl2
• Uniprot:
  • Q62929()