Recombinant Mouse ZNRF3 Fc Chimera Protein, CF


When Recombinant Mouse R‑Spondin 3 (Catalog # 4120-RS) is coated onto a microplate well at 0.25 µg/mL, Recombinant Mouse ZNRF3 Fc Chimera (Catalog # 8328-RF) binds with an apparent Kd <0.2 nM.
1 μg/lane ofRecombinant Mouse ZNRF3(Catalog # 8328-RF)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by silver staining, showing bands at 54.7 and138 kDa, respectively.

Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse ZNRF3 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse R‑Spondin 3 (Catalog # 4120-RS) is coated at 0.25 µg/ml, Recombinant Mouse ZNRF3 Fc Chimera binds with an apparent Kd <0.2 nM.
Mouse myeloma cell line, NS0-derived mouse ZNRF3 protein
Mouse ZNRF3
Accession # Q5SSZ7
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
51-60 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ZNRF3 Fc Chimera Protein, CF

  • BK747E2.3
  • KIAA1133
  • novel C3HC4 type Zinc finger (ring finger)
  • RNF203
  • zinc and ring finger 3
  • zinc/RING finger protein 3
  • ZNRF3


ZNRF3 (zinc/RING finger protein 3) is a member of the ZNRF3 family of type 1 transmembrane ubiquitin ligases (1). Mature mouse ZNRF3 consists of a 163 amino acid (aa) extracellular domain (ECD) with a protease associated (PA) domain fold, a short transmembrane segment, and a 675 aa cytoplasmic tail that contains a RING-type zinc finger with E3 ubiquitin ligase activity. Within the ECD, mouse and human ZNRF3 share 98% sequence identity. ZNRF3 is co-expressed on the cell surface with the homologous protein RNF43. Both proteins serve to inhibit the Wnt signaling pathway through the ubiquitination of LRP6 and a majority of the Frizzled family of Wnt receptors (1, 4, 6). ZNRF3 ubiquitin ligase activity is regulated jointly by R-Spondin and its cell surface receptors, Lgr4, 5, and 6 (2). The R-Spondin-Lgr complex binds to and facilitates the removal of the ZNRF3 from the plasma membrane, resulting in an enhancement of Wnt signaling (3, 4). Conversely, ZNRF3 can antagonize R-Spondin enhanced Wnt signaling (3, 5). ZNRF3 is highly expressed in crypt stem cells of the intestine where it modulates Wnt-induced cell proliferation to control the turnover of intestinal epithelial cells (6). ZNRF3 expression is down-regulated in gastric carcinomas, and ZNRF3 mutations are linked to carcinomas of the gastric tract, pancreas, liver, and ovary (7-10).
  1. de Lau, W. et al. (2014) Genes Dev. 28:305.
  2. Xie, Y. et al. (2013) EMBO Rep. 14:1120.
  3. Peng, W.C. et al. (2013) PLoS One 8:e83110.
  4. Hao, H.X. et al. (2012) Nature 485:195.
  5. Moad, H.E. and A.A. Pioszak (2013) Biochemistry 52:7295.
  6. Koo, B.K. et al. (2012) Nature 488:665.
  7. Ong, C.K. et al. (2012) Nat. Genet. 44:690.
  8. Zhou, Y. et al. (2013) J. Mol. Histol. 44:555.
  9. Ryland, G.L. et al. (2013) J. Pathol. 229:469.
  10. Jiang, X. et al. (2013) Proc. Natl. Acad. Sci. USA 110:12649.

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Gene Symbol Znrf3