Recombinant Mouse YM1/Chitinase 3-like 3 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse YM1/Chitinase 3-like 3 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 5 x 10⁴ cells/well are added to Recombinant Mouse YM1/Chitinase 3-like 3 coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.3-1.2 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse YM1/Chitinase 3-like 3 protein
  Met1-Tyr398, with a C-terminal 6-His tag
• Accession #: NP_034022
• N-terminal Sequence: Tyr22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Chil3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 43 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse YM1/Chitinase 3-like 3 Protein, CF

• AI505981
• CHI3L3
• Chitinase 3 like 3
• Chitinase 3-like 3
• ECF-L
• YM1

Background

Mouse Chitinase 3-like 3 (CHI3L3), also known as ECF-L (eosinophil chemotactic factor‑lymphocyte) or Ym1, is a secreted ~45 kDa glycoprotein that is a member of the glycosyl hydrolase family 18 (chitinase-like) protein family (1-4). Mouse CHI3L3 has no ortholog in humans, but shares 80% and 90% amino acid (aa) sequence identity with rat CHI3L3 and mouse CHI3L4 (also known as Ym2), respectively (3). Mouse CHI3L3 and CHI3L4 share partial overlap in cell type and tissue mRNA expression (2, 5). CHI3L3 is primarily secreted by alveolar and peritoneal macrophages during inflammation, for example, due to nematode infection and airway hyper-responsiveness (2-10). It is considered a marker for alternatively activated macrophages, and is also expressed in bone marrow myeloid precursors, activated microglia, bone marrow-derived immature mast cells, connective tissue-type mast cells, macrophages in erythroblastic islands, neutrophil granules in bone marrow, peritoneum and spleen red pulp, and IL‑4‑stimulated B cells and dendritic cells (DC) (2-13). Statins (cholesterol-lowering drugs) can up‑regulate CHI3L3 expression in DC and promote Th2 responses (11). CHI3L3 can form crystals when it is at high concentration in the macrophage cytoplasm (2, 3, 12, 13). Reports differ as to its enzymatic and binding properties, but CHI3L3 binding of chitin, GlcN polymer or heparin/heparan sulfate may be weak, and later studies do not indicate significant CHI3L3 chemotactic or enzymatic activity (3-5, 9, 12, 13). However, it is proposed to up‑regulate the adhesion molecules LFA‑1 (integrin alpha L beta 2) and ICAM-1, thus promoting cell-cell adhesion (14). CHI3L3 has also shown activity as a co-factor with RANKL or vitamin D in stimulating osteoclast differentiation (14, 15).

1. Owhashi, M. et al. (2000) J. Biol. Chem. 275:1279.
2. Nio, J. et al. (2004) Histochem. Cell Biol. 121:473.
3. Tsai, M-L. et al. (2004) J. Struct. Biol. 148:290.
4. Chang, N.A. et al. (2001) J. Biol. Chem. 276:17497.
5. Webb, D.C. et al. (2001) J. Biol. Chem. 276:41969.
6. Hung, S-L. et al. (2002) J. Leucoc. Biol. 72:72.
7. Raes, G. et al. (2002) J. Leucoc. Biol. 71:597.
8. Nair, M.G. et al. (2005) Infect. Immun. 73:385.
9. Iwashita, H. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:103.
10. Welch, J.S. et al. (2002) J. Biol. Chem. 277:42821.
11. Arora, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7777.
12. Harbord, M. et al. (2002) J. Biol. Chem. 277:5468.
13. Guo, L. et al. (2000) J. Biol. Chem. 275:8032.
14. Garcia-Palacios, V. et al. (2007) Bone 40:316.
15. Oba, Y. et al. (2003) J. Bone Miner. Res. 18:1332.

Publications for YM1/Chitinase 3-like 3 (2446-CH)(3)

Publications using 2446-CH

• SC Starossom, J Campo Garc, T Woelfle, S Romero-Sua, M Olah, F Watanabe, L Cao, A Yeste, JJ Tukker, FJ Quintana, J Imitola, F Witzel, D Schmitz, M Morkel, F Paul, C Infante-Du, SJ Khoury Chi3l3 induces oligodendrogenesis in an experimental model of autoimmune neuroinflammation Nat Commun, 2019-01-15;10(1):217. 2019-01-15 [PMID: 30644388] (Bioassay, Mouse)
• TE Sutherland, D Rückerl, N Logan, S Duncan, TA Wynn, JE Allen Ym1 induces RELM? and rescues IL-4R? deficiency in lung repair during nematode infection PLoS Pathog., 2018-11-30;14(11):e1007423. 2018-11-30 [PMID: 30500858] (In Vivo, Mouse)
• S Wan, X Sun, F Wu, Z Yu, L Wang, D Lin, Z Li, Z Wu, X Sun Chi3l3: a potential key orchestrator of eosinophil recruitment in meningitis induced by Angiostrongylus cantonensis J Neuroinflammation, 2018-02-02;15(1):31. 2018-02-02 [PMID: 29391024] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Chil3
• Uniprot:
  • NP_034022(Mouse)