Recombinant Mouse SLITRK6 Protein, CF

2 μg/lane of Recombinant Mouse SLITRK6 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 80-90 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SLITRK6 Protein, CF Summary

• Additional Information: His-tag
• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived mouse SLITRK6 protein
  Met1-Ser607, with a C-terminal 6-His tag
• Accession #: Q8C110
• N-terminal Sequence: Ser20
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SLITRK6 Protein, CF

• FLJ22774
• MGC119595
• MGC119596
• MGC119597,4832410J21Rik
• SLIT and NTRK-like family, member 6
• SLIT and NTRK-like protein 6
• slit and trk like gene 6
• SLITRK6

Background

SLITRK6 (Slit and Trk-like family member 6) is a type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and a Trk-like cytoplasmic region. Mature mouse SLITRK6 consists of a 587 amino acid (aa) extracellular domain (ECD) with 5 leucine rich repeats (LRR) flanked by N-and C-terminal LRR domains, followed by 6 more LRR flanked by another pair of N- and C-terminal LRR domains (1, 2). Within the ECD, mouse SLITRK6 shares 89% and 95% aa sequence identity with human and rat SLITRK6, respectively. SLITRK6 is strongly expressed in the auditory and vestibular sensory epithelia of the ear (3). Its expression in the inner ear promotes innervation and survival of sensory neurons (3, 4). In the brain, SLITRK6 expression is highly restricted in the thalamus, lateral geniculate nucleus, and absent in the cortex (1, 3). SLITRK6 can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (2, 5). Mice lacking SLITRK6 exhibit delayed retinal synaptogenesis and multiple auditory abnormalities as well as defective auditory sensory innervation, neuronal loss in the vestibular ganglia, and reduced cochlear expression of BDNF, NT-3, TrkB, and TrkC (3, 6, 7). In humans, nonsense mutations of SLITRK6 are associated with myopia and sensorial deafness (6).

1. Aruga, J. et al. (2003) Gene 315:87.
2. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
3. Katayama, K.-I. et al. (2009) PLoS ONE 4:e7786.
4. Morlet, T. et al. (2014) Laryngoscope. 124:95.
5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
6. Tekin, M. et al. (2013) J. Clin. Invest. 123:2094.
7. Matsumoto, Y. et al. (2011) PLoS ONE 6:e16497.

Bioinformatics

• Uniprot:
  • Q8C110()