Recombinant Mouse Reg2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Reg2 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-30 μg/mL on a nitrocellulose-coated microplate.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Reg2 protein
  Gln23-Ala173, with an N-terminal Met
• Accession #: Q08731
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Reg2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Reg2 Protein, CF

• Reg2
• regenerating islet-derived 2
• RegII

Background

Mouse Reg2 (Islets of Langerhans regenerating protein 2), also known as Reg‑II, Lithostathine 2, PTP2 (pancreatic thread protein 2) and PSP2 (pancreatic stone protein 2), is a member of the Reg family of secreted C‑type lectin domain‑containing pancreatic proteins (1, 2). Reg2 forms a subfamily with mouse and human Reg1 molecules (1). The mouse Reg2 cDNA encodes 173 amino acids (aa) including the 22 aa signal sequence and the 151 aa secreted mature protein. Mouse Reg2 shares approximately 83% aa sequence identity with hamster Reg2, but no Reg2 ortholog exists in humans or rats (1, 2). Reg proteins are thought to be stress response proteins that stimulate proliferation, particularly in beta cells of pancreatic islets (1). Reg2 is expressed primarily by acinar cells in the normal exocrine pancreas, and is up‑regulated in NOD (non-obese diabetic) islets, hyperplastic islets, regenerating islets or in response to pancreatic injury or pancreatitis (1‑5). IFN‑ beta causes Reg2 up‑regulation in early‑onset diabetic NOD mice or in a NOD insulinoma cell line (4). Reg2 is also up‑regulated in mice lacking the pancreas‑expressed keratin K8, and is thought to be regulated by keratin filament organization (3). It is proposed to serve as an autoantigen in NOD mouse immune‑mediated type I diabetes models (4).

1. Liu, J.-L. et al. (2008) Endocrine Metab. Immune Disord. Drug Targets 8:1.
2. Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
3. Zhong, B. et al. (2007) Mol. Biol. Cell 18:4969.
4. Gurr, W. et al. (2007) Diabetes 56:34.
5. Lu, Y. et al. (2006) Am. J. Physiol. Endocrinol. Metab. 291:E50.
6. Baeza, N. et al. (1997) FEBS Lett. 416:364.
7. Planas, R. et al. (2006) Diabetologia 49:2379.

Publications for Reg2 (2098-RG)(1)

Publication using 2098-RG

• Zhu, S;Waeckel-Énée, E;Moser, A;Bessard, MA;Roger, K;Lipecka, J;Yilmaz, A;Bertocci, B;Diana, J;Saintpierre, B;Guerrera, IC;Francesconi, S;Mauvais, FX;van Endert, P; Pancreatic islet cell stress induced by insulin-degrading enzyme deficiency promotes islet regeneration and protection from autoimmune diabetes bioRxiv : the preprint server for biology 2023-07-20 [PMID: 37503145] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Reg2
• Uniprot:
  • Q08731()