Recombinant Mouse R-Spondin 3 Protein


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Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse R-Spondin 3 Protein Summary

Details of Functionality
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.25-1 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 3 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
37-43 kDa, reducing conditions
Read Publications using
4120-RS in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse R-Spondin 3 Protein

  • Cristin 1
  • FLJ14440
  • hPWTSR
  • hRspo3
  • Protein with TSP type-1 repeat
  • Roof plate-specific spondin-3
  • RSPO3
  • R-spondin 3 homolog (Xenopus laevis)
  • RSpondin 3
  • R-Spondin 3
  • R-spondin-3
  • Thrombospondin type-1 domain-containing protein 2
  • thrombospondin, type I, domain containing 2
  • THSD2


R-Spondin 3 (RSPO3, roof plate-specific spondin 3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is an ~31 kDa secreted protein that shares ~40% aa identity with the other three R‑Spondin family members (1, 2). All are positive modulators of Wnt/ beta -catenin signaling, but each has a distinct expression pattern (1-4). Like other R‑spondins, R‑Spondin 3 contains two adjacent cysteine-rich furin‑like domains (amino acids (aa) 35-135) with one potential N‑glycosylation site (aa 36), followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-269). Only the furin-like domains are needed for beta -catenin stabilization (2). Within aa 21-209, mouse R‑Spondin 3 shares 93%, 97%, 96%, 95% and 91% aa identity with human, rat, equine, bovine and canine R‑Spondin 3, respectively. Potential isoforms of 217, 224 and 252 aa are divergent or truncated at the C terminus; the 252 aa form also lacks aa 4‑33 at the N‑terminus (5). Mouse R‑Spondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is also essential for expression of the placenta-specific transcription factor, Gcm1. In the mouse embryo, R‑Spondin 3 is often expressed by or located near endothelial cells (6). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (4, 7). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK‑1‑mediated internalization (8, 9). Reports differ on whether R‑Spondins bind LRP-6 directly (8-10). R‑Spondin 3 has also been identified as an oncogene (11).

  1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  2. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
  3. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
  4. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  5. Entrez Accession # BAB28811, BAC36296 and EDL04842.
  6. Kazanskaya, O. et al. (2008) Development 135:3655.
  7. Aoki, M. et al. (2007) Dev. Biol. 301:218.
  8. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  9. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  10. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  11. Theodorou, V. et al. (2007) Nat. Genet. 6:759.

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Publications for R-Spondin 3 (4120-RS)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, In Vivo, Western Blot.

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Showing Publications 1 - 9 of 9.
Publications using 4120-RS Applications Species
KK Dickinson, LC Hammond, CM Karner, ND Hastie, TJ Carroll, P Goodyer Molecular determinants of WNT9b responsiveness in nephron progenitor cells PLoS ONE, 2019;14(4):e0215139. 2019 [PMID: 30978219]
R Ogasawara, D Hashimoto, S Kimura, E Hayase, T Ara, S Takahashi, H Ohigashi, K Yoshioka, T Tateno, E Yokoyama, K Ebata, T Kondo, J Sugita, M Onozawa, T Iwanaga, T Teshima Intestinal Lymphatic Endothelial Cells Produce R-Spondin3 Sci Rep, 2018;8(1):10719. 2018 [PMID: 30013036] (Western Blot) Western Blot
G Greicius, Z Kabiri, K Sigmundsso, C Liang, R Bunte, MK Singh, DM Virshup PDGFR? + pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29559533] (Bioassay, Human) Bioassay Human
S Liu, Y Yin, R Yu, Y Li, W Zhang R-spondin3-LGR4 signaling protects hepatocytes against DMOG-induced hypoxia/reoxygenation injury through activating ?-catenin Biochem. Biophys. Res. Commun., 2018;499(1):59-65. 2018 [PMID: 29555474] (Bioassay, Mouse) Bioassay Mouse
Y Yu, AH Moberly, JP Bhattarai, C Duan, Q Zheng, F Li, H Huang, W Olson, W Luo, T Wen, H Yu, M Ma The Stem Cell Marker Lgr5 Defines a Subset of Post-Mitotic Neurons in the Olfactory Bulb J. Neurosci., 2017;0(0):. 2017 [PMID: 28847812] (Bioassay, Mouse) Bioassay Mouse
X Yin, H Yi, L Wang, W Wu, X Wu, L Yu RSPOs facilitated HSC activation and promoted hepatic fibrogenesis Oncotarget, 2016;7(39):63767-63778. 2016 [PMID: 27572318] (Bioassay, Mouse) Bioassay Mouse
Cambier L, Plate M, Sucov H, Pashmforoush M Nkx2-5 regulates cardiac growth through modulation of Wnt signaling by R-spondin3. Development, 2014;141(15):2959-71. 2014 [PMID: 25053429] (Bioassay, Mouse) Bioassay Mouse
Li, Ji-Yao, Chai, Biaoxin, Zhang, Weizhen, Fritze, Danielle, Zhang, Chao, Mulholland, Michael LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats. Endocrinology, 2014;155(2):429-40. 2014 [PMID: 24280058] (In Vivo, Mouse) In Vivo Mouse
Luo W, Rodriguez M, Valdez J, Zhu X, Tan K, Li D, Siwko S, Xin L, Liu M Lgr4 is a key regulator of prostate development and prostate stem cell differentiation. Stem Cells, 2013;31(11):2492-505. 2013 [PMID: 23897697] (Bioassay, Mouse) Bioassay Mouse

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Gene Symbol Rspo3