Recombinant Mouse Podocan Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Podocan Protein, CF Summary

Details of Functionality
Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. 5 µg/mL of rmPodocan can significantly retard the rate of type I collagen fibrillogenesis.
Source
Mouse myeloma cell line, NS0-derived mouse Podocan protein
Val24-Arg611, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Val24
Structure / Form
Monomer
Protein/Peptide Type
Recombinant Proteins
Gene
Podn
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
67.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
86-92 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Podocan Protein, CF

  • PCAN
  • PODN
  • podocan proteoglycan
  • Podocan
  • SLRR5A
  • SLRR5AMGC24995

Background

Mouse Podocan is a 95 kDa, secreted glycoprotein that is a class V member of the small leucine rich protein gene family (1, 2). It is synthesized as a 611 amino acid (aa) precursor that contains a 23 aa signal sequence, a cysteine-rich region, a series of leucine rich repeats (LRRs), and an extensive acidic C-terminal domain (4, 5). The 15 aa cysteine-rich region (aa 69 - 84) shows a CX3CXCX7C motif, qualifying it as a class V SLRP family member. This is followed by twenty LRRs, thirteen of which are type T (4xLeu; 1xPhe) and seven type S (4xLeu; 2xPro). The LRRs run uninterrupted from aa 89 ‑ 559. The C-terminal seventeen amino acids contain fourteen Glu residues. The negative charge associated with these residues may play a role in basement membrane permeability (4). Mature mouse Podocan is 93% and 94% aa identical to human and canine Podocan, respectively. Over the last 504 aa, mouse Podocan is 98% aa identical to rat Podocan. Podocan is apparently secreted by podocytes and vascular endothelial cells, and deposited in the underlying basement membrane (4). Podocan is known to bind to type I collagen, and have an inhibitory effect on the migration of Podocan-transfected CHO cells (5). The significance of this is unclear.

  1. Matsushima, N. et al. (2000) Proteins 38:210.
  2. Matsushima, N. et al. (2004) Proteins 54:394.
  3. Ross, M.D. et al. (2005) Cell. Mol. Life Sci. 62:2771.
  4. Ross, M.D. et al. (2003) J. Biol. Chem. 278:33248.
  5. Shimizu-Hirota, R. et al. (2004) FEBS Lett. 563:69.

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Bioinformatics

Gene Symbol Podn
Entrez
Uniprot