Recombinant Mouse Podocan Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Podocan Protein, CF Summary

• Details of Functionality: Measured by its ability to modulate collagen fibrillogenesis. Ge, G. et al. (2004) J. Biol. Chem. 279:41626. 5 µg/mL of rmPodocan can significantly retard the rate of type I collagen fibrillogenesis.
• Source: Mouse myeloma cell line, NS0-derived mouse Podocan protein
  Val24-Arg611, with a C-terminal 6-His tag
• Accession #: Q7TQ62
• N-terminal Sequence: Val24
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Podn
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 67.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 86-92 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Podocan Protein, CF

• PCAN
• PODN
• podocan proteoglycan
• Podocan
• SLRR5A
• SLRR5AMGC24995

Background

Mouse Podocan is a 95 kDa, secreted glycoprotein that is a class V member of the small leucine rich protein gene family (1, 2). It is synthesized as a 611 amino acid (aa) precursor that contains a 23 aa signal sequence, a cysteine-rich region, a series of leucine rich repeats (LRRs), and an extensive acidic C-terminal domain (4, 5). The 15 aa cysteine-rich region (aa 69 - 84) shows a CX3CXCX7C motif, qualifying it as a class V SLRP family member. This is followed by twenty LRRs, thirteen of which are type T (4xLeu; 1xPhe) and seven type S (4xLeu; 2xPro). The LRRs run uninterrupted from aa 89 ‑ 559. The C-terminal seventeen amino acids contain fourteen Glu residues. The negative charge associated with these residues may play a role in basement membrane permeability (4). Mature mouse Podocan is 93% and 94% aa identical to human and canine Podocan, respectively. Over the last 504 aa, mouse Podocan is 98% aa identical to rat Podocan. Podocan is apparently secreted by podocytes and vascular endothelial cells, and deposited in the underlying basement membrane (4). Podocan is known to bind to type I collagen, and have an inhibitory effect on the migration of Podocan-transfected CHO cells (5). The significance of this is unclear.

1. Matsushima, N. et al. (2000) Proteins 38:210.
2. Matsushima, N. et al. (2004) Proteins 54:394.
3. Ross, M.D. et al. (2005) Cell. Mol. Life Sci. 62:2771.
4. Ross, M.D. et al. (2003) J. Biol. Chem. 278:33248.
5. Shimizu-Hirota, R. et al. (2004) FEBS Lett. 563:69.

Bioinformatics

• Gene Symbol: Podn
• Uniprot:
  • Q7TQ62()