Recombinant Mouse PILR-beta Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse PILR-beta Protein, CF Summary

• Details of Functionality: Measured by its ability to bind Recombinant Mouse CD99 Fc Chimera (Catalog # 3905-CD) in a functional ELISA. Shiratori, I. et al. (2004) J Exp. Med. 199:525.
• Source: Mouse myeloma cell line, NS0-derived mouse PILR-beta protein
  Ala28-Gly193, with a C-terminal 6-His tag
• Accession #: NP_573472
• N-terminal Sequence: Ala28
• Protein/Peptide Type: Recombinant Proteins
• Gene: Pilrb1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 19.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 39-44 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse PILR-beta Protein, CF

• activating receptor PILRbeta
• Activating receptor PILR-beta
• Cell surface receptor FDFACT
• cell surface receptor FDFACT1
• cell surface receptor FDFACT2
• FDFACT
• FDFACT1
• FDFACT2
• paired immunoglobin-like receptor beta
• paired immunoglobin-like type 2 receptor beta
• paired immunoglobulin-like receptor beta
• paired immunoglobulin-like type 2 receptor beta
• PILRB
• PILRbeta
• PILR-beta

Background

PILR-beta (paired immunoglobulin-like type 2 receptor-beta) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-alpha and it likely arose through PILR-alpha gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-beta and -alpha should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-beta and -alpha show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-beta is a 196 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a short 8 aa cytoplasmic region. The ECD shows a V-type Ig-like domain (aa 39 - 135), while the TM segment contains a positively-charged Lys at position # 202. This Lys is known to interact with the transmembrane signaling adaptor protein DAP12, making PILR-beta an activating receptor. Activation of PILR-beta through CD99 ligation induces NK cell cytotoxicity and dendritic cell secretion of NO and TNF-alpha (3). Mouse PILR-beta is found on NK cells, neutrophils, macrophages, and monocyte-derived dendritic cells (3). Mouse PILR-beta ECD is 44% and 75% aa identical to human and rat PILR-beta ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-alpha (3). Evidence suggests that mouse PILR-beta will not be active in a human system (3). Potential isoforms of PILR-beta have been reported. One shows an alternate start site at Met14, a second shows a 21 aa substitution for the C-terminal 67 aa, and a third exhibits multiple polymorphisms for an overall aa identity of 86% (4, 5, 6).

1. Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
2. Lanier, L.L. (2001) Curr. Opin. Immunol. 13:326.
3. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
4. Genbank Accession # NP_573472.
5. Genbank Accession # BAC29442.
6. Genbank Accession # XP_001480583.

Bioinformatics

• Gene Symbol: Pilrb1
• Uniprot:
  • NP_573472()