Recombinant Mouse MGL2/CD301b Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse MGL2/CD301b Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmMGL2 at 2.5 µg/mL (100 µL/well) on a Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050) coated plate can bind biotinylated beta -Galactosamine-N-Acetyl-Polyacrylamide ( beta -Gal-NAc-PAA) with a linear range of 2-150 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse MGL2/CD301b protein
  Ser72-Pro332, with an N-terminal 10-His tag
• Accession #: NP_660119
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: Mgl2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 31.0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36-38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MGL2/CD301b Protein, CF

• CD301b
• MGL2

Background

Mouse Macrophage Galactose N-acetyl-Galactosamine (GalNAc) specific Lectin 2 (MGL2), also known as CD301b, is a 38 kDa member of the C-type lectin family (1). Two MGL proteins are encoded by separate genes in the mouse, but share 91% amino acid (aa) identity in the extracellular domain (ECD) (1). Only one MGL occurs in human and rat and this MGL is structurally more similar to mouse MGL1 than MGL2. However, human MGL and mouse MGL2 both bind specifically to terminal GalNAc residues, in contrast with mouse MGL1 which binds Lewis X (1, 2). GalNAc recognition is likely to be important in dendritic cell-mediated tolerance to self-gangliosides as well as recognition of tumor antigens and parasite glycoproteins (2). Both mouse MGL proteins are expressed on immature dendritic cells. Mouse MGL2 and MGL1 are markers for connective tissue macrophages of a type termed alternately activated macrophages. These macrophages are induced by IL-4 that is produced during Th2-mediated inflammatory responses to parasitic infections or allergic airway inflammation (3, 4). Quantitative RT-PCR after helminth infection shows increasing expression of MGL2 for at least 29 days while MGL1 shows a peak expression at 7 days (3). This, and data from MGL1 knockout mice,(5) indicates that MGL1 is critical during formulation of granulation tissue, but MGL2 remains involved during chronic infection. Mouse MGL2 is synthesized as a 332 aa type II transmembrane protein with an N-terminal 51 aa cytoplasmic region, a 26 aa TM segment, and a 255 aa ECD. The ECD contains one 150 aa carbohydrate recognition domain (CRD) that shows 76% and 68% aa identity with rat or human MGL, respectively.

1. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
2. Van Vliet, S. J. et al. (2005) Int. Immunol. 17:661.
3. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
4. Sato, K. et al. (2005) Int. Immunol. 17:559.
5. Sato, K. et al. (2005) Blood 106:207.

Bioinformatics

• Gene Symbol: Mgl2
• Uniprot:
  • NP_660119()