Recombinant Mouse ISM1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse ISM1 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived mouse Isthmin 1/ISM1 protein
  Ser27-Tyr461, with a C-terminal 6-His tag
• Accession #: NP_001263418
• N-terminal Sequence: Ser27
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 61-71 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ISM1 Protein, CF

• bA149I18.1
• C20orf82
• Chromosome 20 Open Reading Frame 82
• dJ1077I2.1
• ISM
• ISM1
• ISM-1
• Isthmin 1 Homolog (Zebrafish)
• Isthmin 1 Homolog
• Isthmin 1
• Isthmin
• Isthmin-1

Background

Isthimin 1 (ISM1) is adhesion antiangiogenic molecule found in skin, muscosal tissues and immuneregulatory cells (1). Mature mouse ISM1 contains two key domains, a central 45 aa TSP type 1 domain followed by a C-terminal 164 aa AMOP domain, the latter of which is responsible for much of the antiangiogenic function (2). ISM1 was originally characterized in Xenopus, and mouse ISM1 shares a 77% similarity with its frog counterpart. Much greater conservation is seen across mammalian species, with mouse ISM1 sharing 92% and 98% aa sequence identity with human and rat ISM1, respectively. ISM shares various roles in developing embryos, from craniofacial patterning (3) to development of the lung tissues as well as tissue homeostasis in adults (4). In the vascular system, Isthmin 1 has been described as an angiogenesis inhibitor. It inhibits angiogenesis in vitro and in vivo, as well as during tumor progression (5). A dual function of Isthmin 1 has also been described where it exerts pro-survival effect on endothelial cells when immobilized, whereas soluble Isthmin 1 induced endothelial cell apoptosis (6). Our in-house bioassay has showed that ISM-1 can support cortical neuron neurite outgrowth.

1. Valle-Rios, R. et al. (2014). J. Interf. Cytok. Res. 34:795.
2. Xiang W. et al. (2011). J. Cell. Mol. Med. 15:359.
3. Lansdon, L. et al. (2018). Genetics. 208:283.
4. Osório, L. et al. (2014). Cell cycle. 13:1571.
5. Chen, M. et al. (2014) Cell Death and Differetiation. 21:797.
6. Zhang, Y. et al. (2011) Cell Death. Dis. 2:e153.

Bioinformatics

• Uniprot:
  • NP_001263418()