Recombinant Mouse IL-1Rrp2/IL-1R6 Protein

• Reactivity: Mu
• Applications: Binding Activity

Recombinant Mouse IL-1Rrp2/IL-1R6 Protein Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmIL-1F5, rmIL-1F6, or rmIL-1F8 at 1 µg/mL (100 µL/well) can bind
  rmIL-1 Rrp2/his with a linear range of 0.078-5 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse IL-1 Rrp2/IL-1 R6 protein
  Asp22-Arg338 (Ala158Val), with a C-terminal 6-His tag
• Accession #: Q9ERS7
• N-terminal Sequence: Asp22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il1rl2
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 36.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-68 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-1Rrp2/IL-1R6 Protein

• IL-1 R6
• IL-1 Rrp2
• IL1RL2
• IL-1RL2
• IL1Rrp2
• IL-1Rrp2
• IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2
• interleukin 1 receptor-like 2
• interleukin-1 receptor-like 2

Background

IL-1 Rrp2, also known as IL-1 R6, is a member of the IL-1 R/TLR superfamily. Members of this superfamily have a characteristic Toll-IL-1 R (TIR) domain in their cytoplasmic region. This superfamily can be further divided into three subgroups based on extracellular region analogies. IL-1 Rrp2 belongs to Subgroup 1 which is characterized by the presence of at least one immunoglobulin-like domain (1). Mouse IL-1 Rrp2/IL-1R6 is a 70 kDa type I transmembrane glycoprotein that is synthesized as a 574 amino acid (aa) precursor. The precursor contains a 21 aa signal sequence, a 317 aa extracellular region, a 21 aa transmembrane segment, and a 215 aa cytoplasmic domain (2). The extracellular region contains three C-type Ig-like domains. Within the extracellular region, mouse IL-1 Rrp2 is 64% and 80% aa identical to human and rat IL-1 Rrp2, respectively. Expression of IL-1 Rrp2 is restricted to lung epithelium, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (3). Studies have demonstrated that the functional receptor for IL-1F6, IL-1F8 and IL-1F9 is a combination of IL-1 Rrp2 and IL-1 RAcP. This suggests that IL-1 Rrp2 ligands may signal in a similar fashion to IL-1 and IL-18. In each case, there is a binding receptor which, upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex (4).

1. Dunne, A. and L.A. O’Neill, (2003) Sci. STKE Feb 25 (171):re3.
2. Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
3. Lovenberg, T.S. et al. (1996) J. Neuroimmunol. 70:113.
4. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Bioinformatics

• Gene Symbol: Il1rl2
• Uniprot:
  • Q9ERS7()