Recombinant Mouse G6b Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse G6b Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED₅₀ for this effect is 0.5-2.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse G6b protein
  

  Mouse G6b (Asn18-Leu142) Accession # NP_001028393	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: NP_001028393
• N-terminal Sequence: Asn18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: G6bos
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45-60 kda, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse G6b Fc Chimera Protein, CF

• C6orf25
• chromosome 6 open reading frame 25
• G6b
• immunoglobulin receptor
• MGC142279
• MGC142281
• NG31
• protein G6b

Background

G6b is a type I transmembrane glycoprotein in the immunoglobulin superfamily that is encoded within the human class III major histocompatibility complex (1‑3). It is expressed mainly on platelets, and has also been detected on megakaryocytes and the human cell line K562 (2‑5). Crosslinking of G6b inhibits agonist‑stimulated platelet aggregation and activation (5). The 242 amino acid (aa) mouse G6b (equivalent to human G6b‑B) contains a 17 aa signal sequence, a 123 aa extracellular domain with one V‑type Ig‑like domain and one predicted N‑linked glycosylation site, a 21 aa transmembrane sequence and an 81 aa cytoplasmic sequence with two ITIM inhibitory motifs and an SH3 binding site (1, 2). Phosphorylation at the human equivalent of mouse Y212 within the first ITIM is required for interaction with SHP adaptor/tyrosine phosphatase proteins (2, 3). Of several described human isoforms, all are identical within aa 22‑136, which includes most of the extracellular domain. The one described mouse isoform is the equivalent of human G6b‑A, a transmembrane form with an alternate C‑terminus that lacks the ITIM motifs.  Since the ITIM motifs are required for G6b inhibition of GPIV and CLEC‑2 signals, it is possible that the G6b‑A isoform may be paired with and control inhibition by G6b (4, 6). Heparin interacts with the G6b extracellular domain with high‑affinity and may contribute to G6b activity or adhesion to extracellular matrix (7).

1. Ribas, G. et al. (1999) J. Immunol. 163:278.
2. De Vet, E. C. J. M. et al. (2001) J. Biol. Chem. 276:42070.
3. Senis, Y. A. et al. (2007) Mol. Cell. Proteomics 6:548.
4. Macaulay, I. C. et al. (2007) Blood 109:3260.
5. Newland, S. A. et al. (2007) Blood 109:4806.
6. Mori, J. et al. (2008) J. Biol. Chem. 283:35419.
7. De Vet, E. C. J. M. et al. (2005) FEBS Lett. 579:2355.

Bioinformatics

• Gene Symbol: G6bos
• Uniprot:
  • NP_001028393()