Recombinant Mouse FGF-17 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse FGF-17 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 150-750 ng/mL, in the presence of 10 µg/mL heparin.
Source
E. coli-derived mouse FGF-17 protein
Thr23-Thr216, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Fgf17
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, (NH4)2SO4, DTT and EDTA.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF-17 Protein, CF

  • FGF-13
  • FGF17
  • FGF-17
  • fibroblast growth factor 17

Background

FGF‑17 is a member of the fibroblast growth factor (FGF) family. FGFs play multiple roles in biological functions, including angiogenesis, mitogenesis, cell differentiation and wound repair. FGFs share 30‑70% amino acid (aa) identity in a conserved, approximately 120 amino acid core domain (1‑3). The mouse or human FGF‑17 cDNA encodes a cleavable 22 aa signal sequence and a 194 secreted mature protein (1). Mature mouse FGF-17 shares 100%, 99%, 99%, 97%, and 97% aa identity with rat, human, porcine, canine and equine FGF‑17, respectively. The FGF domain of FGF‑17 shares the most aa identity with FGF-8 (75%) and FGF-18 (64%). These three FGFs constitute a subfamily that overlaps in some areas of expression and function (1‑5). All are reported to bind and signal through FGF R4 and the “c” splice forms of FGF R1-3 (6, 7). During embryogenesis, FGF‑17 plays an organizing and inducing role in the patterning at the midbrain/hindbrain junction, and is also expressed in hindgut, parts of the developing skeleton, tail bud, major arteries, and heart (2‑5). In many of these areas, it is expressed along with FGF-8, but slightly later (2‑6). Unlike FGF-8 and FGF‑18, deletion of FGF‑17 produces viable mice. However, FGF‑17-/- mice show abnormalities in the dorsal frontal cortex, midbrain and cerebellum, manifested in some cases by ataxia, auditory defects, and abnormal social behavior (1, 4, 5, 8, 9). In the adult, FGF-17 is expressed in ovarian follicles and the prostate, and its expression is increased by both benign hypertrophy and cancer of the prostate (10‑12). FGF‑8, FGF‑17, and FGF‑18 are also abnormally expressed in many leukemic cell lines and can enhance growth of cancer cells (13).

  1. Itoh, N. and D.M. Ornitz (2008) Dev. Dyn. 237:18.
  2. Maruoka, Y. et al. (1998) Mech. Dev. 74:175.
  3. Xu, J. et al. (1999) Mech. Dev. 83:165.
  4. Cholfin, J.A. and J.L.R. Rubenstein (2007) Proc. Natl. Acad. Sci. USA 104:7652.
  5. Xu, J. et al. (2000) Development 127:1833.
  6. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
  7. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  8. Yu, X. et al. (2011) Neuroimage 56:1251.
  9. Scearce-Levie, K. et al. (2008) Genes Brain Behav. 7:344.
  10. Machado, M.F. et al. (2009) J. Endocrinol. 202:347.
  11. Polnaszek, N. et al. (2004) Prostate 60:18.
  12. Heer, R. et al. (2004) J. Pathol. 204:578.
  13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.

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Bioinformatics

Gene Symbol Fgf17
Entrez
Uniprot