Recombinant Mouse CXCL15/Lungkine Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse CXCL15/Lungkine Protein Summary

• Details of Functionality: Bioassay data are not available.
• Source: E. coli-derived mouse CXCL15/Lungkine protein
  Gln26-Ala167, with an N-terminal Met
• Accession #: Q9WVL7
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cxcl15
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL15/Lungkine Protein

• CXCL15
• Lungkine
• Scyb15
• Weche

Background

Mouse Lungkine/CXCL15 (1), also named WECHE (2), is a member of the ELR motif-containing CXC chemokines. The cDNA of mouse Lungkine encodes a protein of 167 amino acids (aa) with a 25 aa predicted signal peptide and a 142 aa mature protein with an extremely long C terminal tail that protrudes beyond the chemokine fold. Mouse Lungkine shares 35% aa sequence identity with human ENA-78 and 31% identity with human IL-8. The gene for mouse Lungkine has been mapped to chromosome 5. By Northern blot and in situ hybridization, Lungkine transcripts are only specifically detected in the adult and fetal lung, and its expression is up-regulated under inflammatory conditions (1). Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking (1). Studies from Lungkine knock out mice suggests that Lungkine is an important mediator of neutrophil migration from the lung parenchyma into the airspace (3). Lungkine is also chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation (2).

1. Rossi, D.L. et al. (1999) J. Immunol. 162:5490.
2. Ohneda, O. et al. (2000) Immunity 12:141.
3. Chen, S-C. et al. (2001) J. Immunol. 166:3362.

Publications for CXCL15/Lungkine (442-LK)(5)

Publications using 442-LK

• H Xiong, J Ye, K Xie, W Hu, N Xu, H Yang Exosomal IL-8 derived from Lung Cancer and Colon Cancer cells induced adipocyte atrophy via NF-kappaB signaling pathway Lipids in Health and Disease, 2022-12-29;21(1):147. 2022-12-29 [PMID: 36581870] (Bioassay, Mouse)
• YM Hyun, SU Seo, WS Choi, HJ Kwon, DY Kim, S Jeong, GY Kang, E Yi, M Kim, HJ Ryu, MR Looney, EY Choi, HS Kim Endogenous DEL-1 restrains melanoma lung metastasis by limiting myeloid cell-associated lung inflammation Sci Adv, 2020-11-06;6(45):. 2020-11-06 [PMID: 33158867] (Bioassay, Mouse)
• Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE, 2015-01-28;10(1):e0117908. 2015-01-28 [PMID: 25629162] (Bioassay, Mouse)
• Maheshwari A, Christensen RD, Calhoun DA Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model. Fetal Pediatr Pathol, 2004-03-01;23(2):145-57. 2004-03-01 [PMID: 15768860] (ELISA (Standard))
• Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2004-09-09;105(1):31-9. 2004-09-09 [PMID: 15358618] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Cxcl15
• Uniprot:
  • Q9WVL7()