Recombinant Mouse CD300c Fc Chimera Protein, CF

Recombinant Mouse CD300c Fc Chimera (Catalog # 10183-LM) inhibits anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.8‑4.8 μg/mL.

2 μg/lane of Recombinant Mouse CD300c Fc Chimera (Catalog # 10183-LM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-70 kDa and 110-140 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD300c Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED₅₀ for this effect is 0.8-4.8 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse CD300c protein
  

  Mouse CD300c (His22-Pro187) Accession # A2A7V7	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: A2A7V7
• N-terminal Sequence: His22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-70 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD300c Fc Chimera Protein, CF

• CD300c antigenCMRF35 antigen
• CD300c molecule
• CD300c
• CLM6
• CLM-6
• CMRF35 leukocyte immunoglobulin-like receptor
• CMRF35
• CMRF-35
• CMRF35A leukocyte immunoglobulin-like receptor
• CMRF-35A
• CMRF35ACMRF35A1
• CMRF35CMRF35-A1
• CMRF35-like molecule 6
• IGSF16
• IgSF16
• IGSF16CD300 antigen-like family member C
• Immunoglobulin superfamily member 16
• LIR

Background

CMRF-35-like molecule-6 (CLM-6), known as LMIR8, CMRF-35A1, and CD300c, is a type I transmembrane glycoprotein belonging to the immunoregulatory signaling (IRS) family of immunoglobulin-like molecules (1). There have been 7 human and 9 mouse CD300/CLM family members identified, and they modulate a broad and diverse array of immune cell processes via their paired activating and inhibitory receptor functions (2, 3). Mouse CD300c consists of an extracellular domain (ECD) with one Ig-like domain, a single transmembrane segment, and a short cytoplasmic tail (2). The mature ECD of mouse CD300c is 167 amino acids and shares 48% sequence identity with human CD300c. A charged amino acid residue in the transmembrane domain of CD300c/CLM-6, found in additional family members CLM‑2, CLM-4, CLM-5, and CLM-7, enables interactions with adapter proteins (3,4). CD300c has been shown to be a FcR gamma -coupled receptor that is selectively expressed in Plasmacytoid dendritic cells (pDCs) (5). CD300c signals might negatively regulate the activation of pDCs in response to viral infections (5). Additional research into CD300 receptor function during viral infections could help develop novel anti-viral therapies (6).

1. Clark, G.J. et al. (2001) Tissue Antigens 57:415.
2. Borrego F. (2013) Blood 121:1951.
3. Vitallé J. et al. (2019) Eur J Immunol. 49(3):364.
4. Clark, G.J. et al. (2009) Trends in Immunology 30:209.
5. Kaitani, A. et al. (2018) Scientific Reports 8:8259.
6. Graziano, V et al. 2019. Viruses. 11(6):498.

FAQs for CD300c (10183-LM). (Showing 1 - 1 of 1 FAQ).

1. Why are there different molecular weight bands in western blot images for two of your antibodies (NBP1-84432 and H00010871-B01P) and overexpression lysate NBL1-08931?
   • A variety of band patterns can be expected when detecting CD300C. This protein has multiple glycosliation post-translational modifications that will change its molecular weight, and which vary in different experimental conditions. If you look at the uniprot link listed below, you can view more information on the specific PTMs for CD300C:https://www.uniprot.org/uniprot/Q08708#sequencesWe can be assured that these varying band patterns are indeed detection of CD300C by the absence of bands in the negative control. Any non-specific binding would show in both the negative and positive control, which is not the case here.

Bioinformatics

• Uniprot:
  • A2A7V7()