Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED₅₀ for this effect is 0.3-1.8 μg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse CD300b/LMIR5 protein
  

  Mouse CD300b (Ile18-Tyr157) Accession # Q3U497	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q3U497
• N-terminal Sequence: Ile18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd300lb
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 43.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-58 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF

• CD300 antigen like family member B
• CD300 antigen-like family member B
• CD300 molecule-like family member b
• CD300b antigen
• CD300b
• CD300LB
• CLM7
• CLM-7
• CLM7IREM3CD300 molecule like family member b
• CMRF35A2
• CMRF35-A2
• CMRF35-like molecule 7
• EC 1.13.11.6
• EC 6.3.4.3
• Gm253
• Immune receptor expressed on myeloid cells 3
• IREM-3
• Leukocyte mono-Ig-like receptor 5
• LMIR5
• TREM-5
• TREM5CD300b
• Triggering receptor expressed on myeloid cells 5

Background

LMIR5, also known as CD300b, CD300LB, CLM-7, and IREM‑3, is a 26 kDa-32 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse LMIR5 consists of a 140 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, mouse LMIR5 shares 51% and 86% aa sequence identity with human and rat LMIR5, respectively. The transmembrane segment contains a positively charged lysine which enables the association of LMIR5 with DAP12, DAP10, and potentially other adaptor proteins (2, 3). The cytoplasmic domain of human LMIR5 contains a phosphorylatable tyrosine motif, while that of mouse LMIR5 does not (2). LMIR5 is expressed on the surface of myeloid lineage cells (2-4). It forms noncovalent cis‑homodimers and cis-heterodimers with other CD300 family proteins, and the composition of these dimers affects the cellular response (2, 5). Antibody cross‑linking of LMIR5 induces mast cell granule release and cytokine production as well as its tyrosine phosphorylation of LMIR5 (in human) (2, 3). LMIR5 interacts with TIM1 and TIM4 which regulate T cell activation and are themselves binding partners (6). TIM1 interactions with LMIR5 mediate mast cell activation and the accumulation of neutrophils at sites of TIM1 up‑regulation on damaged renal tubule epithelial cells (6).

1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
2. Martinez-Barriocanal, A. et al. (2006) J. Immunol. 177:2819.
3. Yamanishi, Y. et al. (2008) Blood 111:688.
4. Wu, Y. et al. (2011) Cell. Immunol. 268:68.
5. Martinez-Barriocanal, A. et al. (2010) J. Biol. Chem. 285:41781.
6. Yamanishi, Y. et al. (2010) J. Exp. Med. 207:1501.

Publications for CD300b/LMIR5/CD300LB (2580-LM)(1)

Publication using 2580-LM

• A soluble form of LMIR5/CD300b amplifies lipopolysaccharide-induced lethal inflammation in sepsis. J. Immunol., 2012-07-06;189(4):1773-9. 2012-07-06 [PMID: 22772446] (ELISA (Standard))

Bioinformatics

• Gene Symbol: Cd300lb
• Uniprot:
  • Q3U497()