Recombinant Human ZNRF3 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ZNRF3 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human R‑Spondin 3 (Catalog # 3500-RS) is coated at 0.25 μg/mL, Recombinant Human ZNRF3 Fc Chimera binds with an apparent K_D <3 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived human ZNRF3 protein
  

  Human ZNRF3 (Lys56-Met219) Accession # Q9ULT6	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9ULT6
• N-terminal Sequence: Lys56
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: ZNRF3
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 44.8 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ZNRF3 Fc Chimera Protein, CF

• BK747E2.3
• KIAA1133
• novel C3HC4 type Zinc finger (ring finger)
• RNF203
• zinc and ring finger 3
• zinc/RING finger protein 3
• ZNRF3

Background

ZNRF3 (zinc/RING finger protein 3), also called RNF203, is a 101 kDa (predicted) type I transmembrane protein that is a member of the ZNRF3 family of ubiquitin ligases (1). Human ZNRF3 is synthesized as a 936 amino acid (aa) protein that contains a putative 55 aa signal sequence, a 164 aa extracellular domain (ECD), a transmembrane domain, and a cytoplasmic domain with an atypical RING (really interesting new gene) -type zinc finger (1). The mature human ZNRF3 ECD shares 98% aa sequence identity with mouse ZNRF3. An alternative start site at Met101 creates a 90 kDa (predicted) form; the shorter ECD of this human isoform shares 100% aa sequence identity with canine, feline and equine, 99% with porcine, and 98% with rat ZNRF3 (1). In the plasma membrane, ZNRF3 is co‑expressed with the related protein RNF43, and both serve as E3 ubiquitin ligases that promote turnover of Frizzled Wnt receptors and LRP6 to antagonize Wnts (2‑4). Interaction with the R‑Spondin Wnt‑regulating proteins antagonizes ZNRF3, thus stabilizing Frizzleds and LRP6, allowing their accumulation, and promoting Wnt signaling (2, 3). ZNRF3 is expressed in stem cells at the bottom of colon crypts, where it limits the ability of Wnts to induce proliferation (4). It is also expressed in normal gastric tissues, but is down‑regulated in gastric adenocarcinomas (5).

1. SwissProt Accession # Q9ULT6.
2. MacDonald, B.T. and X. He (2012) Cell Res. 22:1410.
3. Hao, H.X. et al. (2012) Nature 485:195.
4. Koo, B.K. et al. (2012) Nature 488:665.
5. Zhou, Y. et al. (2013) J. Mol. Histol. Mar 17 [Epub ahead of print].

Publications for ZNRF3 (7994-RF)(1)

Publication using 7994-RF

• H Lee, C Seidl, R Sun, A Glinka, C Niehrs R-spondins are BMP receptor antagonists in Xenopus early embryonic development Nat Commun, 2020-11-04;11(1):5570. 2020-11-04 [PMID: 33149137] (Bioassay, Human)

Bioinformatics

• Gene Symbol: ZNRF3
• Uniprot:
  • Q9ULT6()