Recombinant Human SLITRK6 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SLITRK6 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human SLITRK6 protein
  Ile16-Ala604, with a C-terminal 6-His tag
• Accession #: Q9H5Y7
• N-terminal Sequence: Ile16 & His19
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 72-95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SLITRK6 Protein, CF

• FLJ22774
• MGC119595
• MGC119596
• MGC119597,4832410J21Rik
• SLIT and NTRK-like family, member 6
• SLIT and NTRK-like protein 6
• slit and trk like gene 6
• SLITRK6

Background

SLITRK6 (Slit and Trk-like family member 6) is a type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region. Mature human SLITRK6 consists of a 582 amino acid (aa) extracellular domain (ECD) with 5 leucine rich repeats (LRR) flanked by N-and C-terminal LRR domains, followed by 6 more LRR flanked by another pair of N- and C-terminal LRR domains (1, 2). Within the ECD, human SLITRK6 shares 88% aa sequence identity with mouse and rat SLITRK6. SLITRK6 is expressed in multiple regions of the developing nervous system, notably in the otic vesicle, cochlear and vestibular sensory epithelia, and spiral and vestibular ganglion neurons (3, 4). It can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (2, 5). Mice lacking SLITRK6 exhibit delayed retinal synaptogenesis and multiple auditory abnormalities as well as defective auditory sensory innervation, neuronal loss in the vestibular ganglia, and reduced cochlear expression of BDNF, NT-3, TrkB, and TrkC (4, 6, 7). In humans, nonsense mutations of SLITRK6 are associated with myopia and sensorial deafness (6).

1. Aruga, J. et al. (2003) Gene 315:87.
2. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
3. Aruga, J. (2003) Gene Expr. Patterns 3:727.
4. Katayama, K.-I. et al. (2009) PLoS ONE 4:e7786.
5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
6. Tekin, M. et al. (2013) J. Clin. Invest. 123:2094.
7. Matsumoto, Y. et al. (2011) PLoS ONE 6:e16497.

Bioinformatics

• Uniprot:
  • Q9H5Y7(Human)