Recombinant Human SLITRK2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SLITRK2 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human SLITRK2 protein
  Thr19-Pro617, with a C-terminal 6-His tag
• Accession #: Q9H156
• N-terminal Sequence: Thr19
• Protein/Peptide Type: Recombinant Proteins
• Gene: SLITRK2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SLITRK2 Protein, CF

• CXorf2
• SLITL1
• SLITRK2 SLIT and NTRK-like family, member 2
• SLITRK2

Background

SLITRK2 (Slit and Trk-like family member 2) is an approximately 110 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature human SLITRK2 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, human SLITRK2 shares 98% amino acid sequence identity with mouse and rat SLITRK2. SLITRK2 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). It can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (3-5). The synaptogenic function is dependent on the interaction of SLITRK2 with select isoforms of PTP-sigma (5, 6). In humans, mutations of SLITRK6 are associated with bipolar disorder and schizophrenia (7, 8).

1. Ko, J. (2012) Mol. Cells 34:335.
2. Aruga, J. et al. (2003) Gene 315:87.
3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
6. Yamagata, A. et al. (2015) Sci. Rep. 5:9686.
7. Smith, E.N. et al. (2009) Mol. Pschiatry 14:755.
8. Piton, A. et al. (2011) Mol. Pschiatry 16:867.

Bioinformatics

• Gene Symbol: SLITRK2
• Uniprot:
  • Q9H156()