Recombinant Human SALM3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SALM3 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. When 5 x 10⁴ cells/well are added to Recombinant Human SALM3/LRFN4 coated plates (2.5 μg/mL, 100 μL/well). approximately 50%-70% will adhere after 30 minutes at 37 °C.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human SALM3/LRFN4 protein
  Cys17-Leu518 with a C-terminal 6-His tag
• Accession #: Q6PJG9
• N-terminal Sequence: Cys17
• Protein/Peptide Type: Recombinant Proteins
• Gene: LRFN4
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 54.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SALM3 Protein, CF

• FIGLER6
• LRFN4
• SALM3
• SALM3.

Background

Synaptic adhesion-like molecule 3 (SALM3; also leucine-rich repeat and fibronectin type-III domain-containing protein 4 (Lrfn4) is an approximately 90 kDa member of the Lrfn family of type I transmembrane glycoproteins (1).  Human SALM3 is synthesized as a 635 amino acid (aa) precursor that contains a 16 aa signal sequence, a 502 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 96 aa cytoplasmic region.  The ECD consists of seven leucine-rich repeats (LRR), an IgC2‑like domain, and a fibronectin type-III domain, tandemly aligned in that order (1-2).  In addition, there are six potential sites for N-linked glycosylation. The C‑terminal region contains an intracellular PDZ binding domain, which is conserved among SALMs 1-3, but is absent in SALMs 4 and 5 (3). Mature human SALM3 shares 97% aa sequence identity with mature mouse SALM3.  Northern blot analysis showed that in mice, SALM3 is strongly expressed in the adult brain and is also present in the adult testis (1). It is distributed throughout the neuron, including the growth cone (3).  In the developing mouse embryo, a temporal expression profile blot revealed a general increment of expression around E10.5, with weak expression detected before E10.5 (1). SALM3, like the other SALMs, promotes neurite outgrowth (3).  Specifically, the SALMs modify total outgrowth and neurite branching (3).  SALM3 may also be involved in synapse formation, synaptic maintenance, and other cellular interactions (3).

1. Morimura, N. et al. (2006) Gene 380:72.
2. Wang, C.-Y. et al. (2006) J. Neurosci. 26:2174.
3. Wang, P.Y. et al. (2008) Mol. Cell. Neurosci. 39:83.

Bioinformatics

• Gene Symbol: LRFN4
• Uniprot:
  • Q6PJG9(Human)