Recombinant Human Reg1B Protein, CF

Recombinant Human Reg1B (Catalog # 2090-RG) stimulates cell proliferation in RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.15-0.75 μg/mL.

1 μg/lane ofRecombinant Human Reg1B (Catalog # 2090-RG)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by silver staining, showing bands at 17.2 and 16.7 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Reg1B Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED₅₀ for this effect is 0.15-0.75 μg/mL.
• Source: E. coli-derived human Reg1B protein
  Gln23-Asn166, with an N-terminal Met
• Accession #: P48304
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: S100A9
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 16 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Reg1B Protein, CF

• Lithostathine 1 beta
• lithostathine-1-beta
• Pancreatic stone protein 2
• pancreatic threadprotein)
• PSPS2
• PSPS2PSP-2
• Reg1B
• regenerating islet-derived 1 beta
• Regenerating islet-derived protein 1-beta
• Regenerating protein I beta
• REGH
• REGLREG-1-beta
• secretory pancreatic stone protein 2

Background

Reg1B also called Lithostatine-1-beta or Pancreatic stone protein 2 (PSP2) is a type I subclass member of the Reg gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins of the genes. Reg1B is also a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). The human Reg1B cDNA encodes 166 amino acids protein which contains a 22 aa signal peptide and the 144 aa secreted mature protein. Reg1B shares 86% animo acide sequence identity with Reg1A and like Reg1A is secreted by the exocrine pancreas and associated with islet cell regeneration and diabetogenesis. Reg family proteins were also shown to cause Schwann cell proliferation during regeneration of motor neurons (3).

1. Liu, J.-L. et al. (2008) Endocrine Metab. Immune Disord. Drug Targets 8:1.
2. Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
3. Livesey F.J. et al. (1990) Nature. 390:614.

Bioinformatics

• Gene Symbol: S100A9
• Uniprot:
  • P48304()