Recombinant Human Reg1A Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Reg1A Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Reg1A, immobilized at 5 μg/mL, is able to significantly enhance neurite outgrowth.
Source
Human embryonic kidney cell, HEK293-derived human Reg1A protein
Gln23-Asn166
Accession #
N-terminal Sequence
No results obtained. Gln23 inferred from enzymatic pyroglutamate treatment revealing Glu24
Structure / Form

Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17-20 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Reg1A Protein, CF

  • ICRF
  • PSP
  • PTP
  • Reg1A

Background

Reg1A (Regenerating islet-derived 1 alpha), also called Lithostatine-1-alpha or Pancreatic stone protein (PSP), is a secreted, variably glycosylated 15-22 kDa type I member of the REG gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins of the genes. Reg1A is also a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). The human Reg1A cDNA encodes a 166 amino acids (aa) precursor which contains a 22 aa signal peptide and a 144 aa secreted mature protein. Human Reg1A shares 86% aa sequence identity with Reg1B, and shares 76% and 69% aa sequence identity with mouse and rat Reg1A, respectively. Reg1A was first described in cells of digestive systems, but is also expressed in a variety of other tissues including neuronal cell lines (PC12 and Neuro-2a) and in rat primary hippocampal neurons (3, 4). In the digestive system, Reg-1A acts as a paracrine/autocrine factor and plays an important role in cell proliferation, differentiation, inflammation, and carcinogenesis (5, 6). In neurons, Reg-1A is preferentially localized at the membrane and around the nucleus of neuronal cells (4). In addition, Reg-1A is secreted, and it positively regulates neurite outgrowth possibly through the membrane receptor exostosin tumor-like 3 (EXTL3) (4). Reg-1A is overexpressed during the very early stages of Alzheimer disease, and its deposits were detected in the brain of patients with Alzheimer disease (7).
  1. Liu, J.L. et al. (2008) Endocr. Metab. Immune. Disord. Drug Targets 8:1.
  2. Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
  3. De Caro A. et al. (1979) Biochem. Biophys. Res. Commun. 87:1176
  4. Acquatella-Tran Van Ba, I. et al. (2012) J. Biol. Chem. 287:4726.
  5. Zhang Y.W. et al. (2003) World J. Gastroenterol. 9:2635.
  6. Miyaoka Y. et al. (2004) Oncogene 23:3572.
  7. Duplan, L. et al. (2001) Neurobiol. Aging 22:79.

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Bioinformatics

Entrez
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