Recombinant Human QBRICK Fc Chimera Protein, CF


There are currently no images for QBRICK/FREM1 (5100-QB).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human QBRICK Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. Kiyozumi, D. et al. (2005) Exp. Cell Res. 306:9. When 5 x 104 cells/well are added to rhQBRICK/Fc Chimera coated plates (2.5 µg/mL, 100 µL/well), approximately 60%‑80% will adhere after 1 hour at 37° C.
Optimal dilutions should be determined by each laboratory for each application.
Chinese Hamster Ovary cell line, CHO-derived human QBRICK/FREM1 protein
(Ser22 - Glu384)
Accession # Q5H8C1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
67.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
75-85 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human QBRICK Fc Chimera Protein, CF

  • BNAR
  • C9orf143chromosome 9 open reading frame 154
  • C9orf145C9orf154extracellular matrix protein QBRICK
  • C9orf154
  • DKFZp686M16108
  • FLJ25461
  • FRAS1 related extracellular matrix 1
  • FRAS1-related extracellular matrix protein 1
  • FREM1
  • Protein QBRICK


QBRICK, also known as Frem1 (Fras1-related extracellular matrix gene1) is a 244 kDa (predicted), secreted, extracellular matrix glycoprotein and member of the Fras1 family of proteins (1 - 3). Human QBRICK is synthesized as a 2179 amino acid (aa) precursor that has a 21 aa signal sequence and a 2158 aa mature chain (SwissProt #: Q5H8C1). The mature chain is made up of an N-terminal variable region domain containing an Arg-Gly-Asp (RGD) cell adhesive motif, 12 consecutive chondroitin sulfate proteoglycan (CSPG) repeats of approximately 120 aa, a Calx-beta domain, a second RGD sequence, and a C-terminal C-type lectin-like domain, respectively (1). In addition, there are five potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for human QBRICK. Because of the characteristic feature of the 12 CSPG repeats, the protein was named QBRICK: “Q” stands for queen and is taken from the queen being the twelfth in a suit of playing cards, and “BRICK” stands for the repeating unit (1). Human QBRICK shares 78% aa sequence identity with mouse QBRICK. QBRICK is localized to the basement membrane in mesenchymal tissue (3). QBRICK plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. QBRICK mediates cell-substratum adhesion through alpha V or alpha 8 integrins (1 - 4). Mutations in QBRICK and other Fras1 family proteins (i.e. Fras1 and Frem2) are associated with Fraser syndrome, a recessive multiorgan disorder characterized by crypthophthalmos, syndactyly, renal agenesis, and a variety of morphogenetic defects (2 - 4). It is postulated that QBRICK, Fras1 and Frem2 make up a ternary complex that act together to ensure adhesion between the epidermal basement membrane and the underlying mesenchyme in embryonic skin (3).

  1. Kiyozumi, D. et al. (2005) Exp. Cell Res. 306:9.
  2. Smyth, I. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:13560.
  3. Kiyozumi, D. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103:11981.
  4. Smyth, I. and P. Scambler (2005) Hum. Mol. Genet. 14:R269.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IF
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ca, Mk, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Mu
Applications: WB, Simple Western, ELISA, IHC
Species: Hu
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IP, ICC, ICFlow, KO
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, IF
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Ca, Mk
Applications: WB, IHC, IHC-P

Publications for QBRICK/FREM1 (5100-QB) (0)

There are no publications for QBRICK/FREM1 (5100-QB).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for QBRICK/FREM1 (5100-QB) (0)

There are no reviews for QBRICK/FREM1 (5100-QB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for QBRICK/FREM1 (5100-QB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for QBRICK/FREM1 (5100-QB)

Discover related pathways, diseases and genes to QBRICK/FREM1 (5100-QB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for QBRICK/FREM1 (5100-QB)

Discover more about diseases related to QBRICK/FREM1 (5100-QB).

Pathways for QBRICK/FREM1 (5100-QB)

View related products by pathway.

PTMs for QBRICK/FREM1 (5100-QB)

Learn more about PTMs related to QBRICK/FREM1 (5100-QB).


There are no specific blogs for QBRICK/FREM1, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human QBRICK Fc Chimera Protein, CF and receive a gift card or discount.


Gene Symbol FREM1