Recombinant Human PSG6 Protein, CF

Recombinant Human PSG6 (Catalog # 9318-P6) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PSG6 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.9-4.5 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human PSG6 protein
  Gln35-His424, with a C-terminal 6-His tag
• Accession #: Q00889-2
• N-terminal Sequence: No results obtained. Gln35 inferred from enzymatic pyroglutamate treatment revealing Val36
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-73 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PSG6 Protein, CF

• CGM3
• pregnancy specific beta-1-glycoprotein 6
• Pregnancy-specific beta-1-glycoprotein 10
• Pregnancy-specific beta-1-glycoprotein 12
• pregnancy-specific beta-1-glycoprotein 6
• Pregnancy-specific glycoprotein 10
• Pregnancy-specific glycoprotein 12
• Pregnancy-specific glycoprotein 6
• PS-beta-G-10
• PS-beta-G-12
• PS-beta-G-6
• PSBG-10
• PSBG-12
• PSBG-6
• PSG10
• PSG12
• PSG6
• PSG-6
• PSGGB

Background

Pregnancy-specific glycoprotein 6 (PSG6), also known as PSG10 and PSGGB is a secreted glycoprotein of the PSG family within the CEA (carcinoembryonic antigen) superfamily (1). Mature human PSG6 is a 401 amino acid (aa) protein that consists of four tandem immunoglobulin-like domains followed by a 30 aa
C-terminal tail (2, 3). The N-terminal Ig-like domain contains an Arg-Gly-Asp (RGD) sequence which is a consensus integrin-binding motif found in many adhesion proteins (4, 5). Alternative splicing generates an additional isoform with a substitution in the C-terminal tail. PSG6 is predominantly expressed in placenta and more weakly in granulocytes (3). It induces monocyte production of the cytokines IL-6, IL-10, and TGF-beta 1 (6).

1. Kuespert, K. et al. (2006) Curr. Opin. Cell Biol. 18:565.
2. Zimmermann, W. et al. (1989) Biochem. Biophys. Res. Commun. 163:1197.
3. Barnett, T.R. et al. (1990) Biochemistry 29:10213.
4. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
5. Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
6. Snyder, S.K. et al. (2001) Am. J. Reprod. Immunol. 45:205.

Bioinformatics

• Uniprot:
  • Q00889-2()