Recombinant Human Olfactomedin-2/Noelin-2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Olfactomedin-2/Noelin-2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of HEK293T human embryonic kidney cells.

  When 5 x 10⁴ cells per well are added to human Olfactomedin-2 coated plates (1 μg/mL, 100 μL/well), approximately 50%-75% will adhere after 1 hour at 37 °C.

  Optimal dilutions should be determined by each laboratory for each application.

• Source: Chinese Hamster Ovary cell line, CHO-derived human Olfactomedin-2/Noelin-2 protein
  Gln21-Pro454, with a C-terminal 6-His tag
• Accession #: NP_477512
• N-terminal Sequence: No results obtained: Gln21 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: OLFM2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Olfactomedin-2/Noelin-2 Protein, CF

• neuronal olfactomedin related ER localized protein 2
• NOE2
• NOE2noelin 2
• NOELIN2
• Noelin-2
• NOELIN2_V1
• olfactomedin 2
• Olfactomedin2
• Olfactomedin-2
• OlfC
• OLFM2
• OM2

Background

Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum-localized-2 (Noelin-2) is a 50 kDa (predicted), secreted member of the olfactomedin/noelin family of proteins (1 - 3). Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name (4). Olfactomedin domain-containing proteins are often found in neural tissues (3). Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum (1, 2). In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation (5). The human Olfactomedin-2 cDNA encodes a 20 amino acid (aa) signal sequence and a 454 mature protein that contains two coiled-coil regions (aa 58 - 85 and 136 - 193) and an Olfactomedin domain (aa 194 - 446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus, and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2 (1). Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients (6). The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noelin-1 might heterodimerize with Olfactomedin-2 (1). Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication (1).

1. Mukhopadhyay, A. et al. (2004) Mol. Vis. 10:304.
2. Kulkarni, N.H. et al. (2000) Genet. Res. Camb. 76:41.
3. Tomarev, S.I. and N. Nakaya (2009) Mol. Neurobiol. 40:122.
4. Moreno, T.A. & M. Bronner-Fraser (2002) Mech. Dev. 119:121.
5. Lee, J-A. et al. (2008) Mech. Dev. 125:167.
6. Funayama, T. et al. (2006) Invest. Ophthalmol. Vis. Sci. 47:5368.

Bioinformatics

• Gene Symbol: OLFM2
• Uniprot:
  • NP_477512()